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Visan S, Soritau O, Tatomir C, Baldasici O, Balacescu L, Balacescu O, Muntean P, Gherasim C, Pintea A. The Bioactive Properties of Carotenoids from Lipophilic Sea buckthorn Extract ( Hippophae rhamnoides L.) in Breast Cancer Cell Lines. Molecules 2023; 28:molecules28114486. [PMID: 37298962 DOI: 10.3390/molecules28114486] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/04/2023] [Revised: 05/25/2023] [Accepted: 05/30/2023] [Indexed: 06/12/2023] Open
Abstract
In women, breast cancer is the most commonly diagnosed cancer (11.7% of total cases) and the leading cause of cancer death (6.9%) worldwide. Bioactive dietary components such as Sea buckthorn berries are known for their high carotenoid content, which has been shown to possess anti-cancer properties. Considering the limited number of studies investigating the bioactive properties of carotenoids in breast cancer, the aim of this study was to investigate the antiproliferative, antioxidant, and proapoptotic properties of saponified lipophilic Sea buckthorn berries extract (LSBE) in two breast cancer cell lines with different phenotypes: T47D (ER+, PR+, HER2-) and BT-549 (ER-, PR-, HER2-). The antiproliferative effects of LSBE were evaluated by an Alamar Blue assay, the extracellular antioxidant capacity was evaluated through DPPH, ABTS, and FRAP assays, the intracellular antioxidant capacity was evaluated through a DCFDA assay, and the apoptosis rate was assessed by flow cytometry. LSBE inhibited the proliferation of breast cancer cells in a concentration-dependent manner, with a mean IC50 of 16 µM. LSBE has proven to be a good antioxidant both at the intracellular level, due to its ability to significantly decrease the ROS levels in both cell lines (p = 0.0279 for T47D, and p = 0.0188 for BT-549), and at the extracellular level, where the ABTS and DPPH inhibition vried between 3.38-56.8%, respectively 5.68-68.65%, and 35.6 mg/L equivalent ascorbic acid/g LSBE were recorded. Based on the results from the antioxidant assays, LSBE was found to have good antioxidant activity due to its rich carotenoid content. The flow cytometry results revealed that LSBE treatment induced significant alterations in late-stage apoptotic cells represented by 80.29% of T47D cells (p = 0.0119), and 40.6% of BT-549 cells (p = 0.0137). Considering the antiproliferative, antioxidant, and proapoptotic properties of the carotenoids from LSBE on breast cancer cells, further studies should investigate whether these bioactive dietary compounds could be used as nutraceuticals in breast cancer therapy.
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Affiliation(s)
- Simona Visan
- Department of Genetics, Genomics, and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Olga Soritau
- Department of Cell Biology and Radiobiology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Corina Tatomir
- Department of Cell Biology and Radiobiology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Oana Baldasici
- Department of Genetics, Genomics, and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Loredana Balacescu
- Department of Genetics, Genomics, and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Ovidiu Balacescu
- Department of Genetics, Genomics, and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 400015 Cluj-Napoca, Romania
| | - Patricia Muntean
- Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
| | - Cristina Gherasim
- Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
| | - Adela Pintea
- Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
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Coman V, Scurtu VF, Coman C, Clapa D, Iancu ȘD, Leopold N, Leopold LF. Effects of polystyrene nanoplastics exposure on in vitro-grown Stevia rebaudiana plants. PLANT PHYSIOLOGY AND BIOCHEMISTRY : PPB 2023; 197:107634. [PMID: 36965317 DOI: 10.1016/j.plaphy.2023.03.011] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/24/2022] [Revised: 02/28/2023] [Accepted: 03/07/2023] [Indexed: 06/18/2023]
Abstract
Nanoplastics (NPs) as environmental contaminants have received increased attention in recent years. Numerous studies have suggested possible negative effects of plants exposure to NPs, but more data are needed with various plants under different exposure conditions to clarify the underlying phytotoxicity mechanisms. In this study, we investigated the effect of polystyrene nanoplastics (PSNPs; 28.65 nm average diameter) exposure (10, 100 and 250 mg/L) on plant morphology and production of relevant metabolites (steviol glycosides, chlorophylls, carotenoids, and vitamins) of in vitro-grown Stevia rebaudiana plantlets. Additionally, we used dark field microscopy combined with fluorescence hyperspectral imaging for the visualization of internalized PSNPs inside plant tissues. At higher concentrations (>100 mg/L), PSNPs were shown to aggregate in roots and to be transported to leaves, having a significantly negative impact on plant growth (reduced size and biomass), while increasing the production of metabolites compared to controls, most probably because of response to stress. The production of steviol glycosides presented a biphasic dose-response suggestive of hormesis, with the highest values at 10 mg/L PSNPs (1.5-2.2-fold increase compared to controls), followed by a decline in production at higher concentrations (100 and 250 mg/L), but with values comparable to controls. These results are promising for future in vivo studies evaluating the effect of NP exposure on the production of steviol glycosides, the natural sweeteners from stevia.
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Affiliation(s)
- Vasile Coman
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
| | - Violeta-Florina Scurtu
- Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
| | - Cristina Coman
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
| | - Doina Clapa
- Faculty of Horticulture and Business in Rural Development, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
| | - Ștefania D Iancu
- Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084, Cluj-Napoca, Romania.
| | - Nicolae Leopold
- Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084, Cluj-Napoca, Romania.
| | - Loredana-Florina Leopold
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Romania.
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Parvez MK, Al-Dosari MS, Basudan OA, Herqash RN. The anti‑hepatitis B virus activity of sea buckthorn is attributed to quercetin, kaempferol and isorhamnetin. Biomed Rep 2022; 17:89. [PMID: 36185785 PMCID: PMC9500493 DOI: 10.3892/br.2022.1573] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/23/2022] [Accepted: 09/06/2022] [Indexed: 11/05/2022] Open
Abstract
The present study assessed the in vitro anti-hepatitis B virus (HBV) effects of cold-adapted sea buckthorn (Hippophae rhamnoides). Sea buckthorn leaf ethanol extracts subjected to chloroform (SB-Chl), ethyl acetate (SB-Eac), n-butanol (SB-But) and aqueous (SB-Aqu) fractionation were first examined (MTT assay) for their toxic effects on HepG2 cells. While SB-Chl (IC50, 32.58 µg/ml) exhibited high cytotoxicity, SB-Eac, SB-But SB-Aqu were non-toxic at up to 150 µg/ml. High performance liquid chromatography analysis led to the identification of the anti-HBV active flavonols, quercetin (93.09 µg/g), kaempferol (44.19 µg/g) and isorhamnetin (138.75 µg/g) in the extract. The analysis of the anti-HBV effects of SB-Eac, SB-But and SB-Aqu (50 µg/ml, each) on HepG2.2.15 cells revealed the marked inhibition of HBsAg and HBeAg expression levels. At the concentration of 10 µg/ml, quercetin and kaempferol exerted potent inhibitory effects on HBsAg (60.5 and 62.3%, respectively) and HBeAg synthesis (64.4 and 60.2%, respectively), as compared to isorhamnetin (30.5 and 28.4%, respectively). The HBV-polymerase inhibitor drug, lamivudine (2 µM), inhibited HBsAg and HBeAg expression by 87.4 and 83.5%, respectively. The data were in good agreement with a previous in vitro and in silico molecular docking analysis performed by the authors where quercetin, kaempferol and lamivudine had formed stable complexes with HBV-polymerase binding-pocket amino acids. On the whole, to the best of our knowledge, the present study provides the first report of the anti-HBV therapeutic potential of sea buckthorn, attributed to quercetin, kaempferol and isorhamnetin.
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Affiliation(s)
- Mohammad K. Parvez
- Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Mohammed S. Al-Dosari
- Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Omar A. Basudan
- Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
| | - Rashed N. Herqash
- Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
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Gadolinium Accumulation and Toxicity on In Vitro Grown Stevia rebaudiana: A Case-Study on Gadobutrol. Int J Mol Sci 2022; 23:ijms231911368. [PMID: 36232670 PMCID: PMC9569896 DOI: 10.3390/ijms231911368] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2022] [Revised: 09/19/2022] [Accepted: 09/20/2022] [Indexed: 11/17/2022] Open
Abstract
Gadolinium-based contrast agents are molecular complexes which are extensively used for diagnostic purposes. Apart from their tremendous contribution to disease diagnostics, there are several issues related to their use. They are extremely stable complexes and potential contaminants of surface and ground waters, an issue which is documented worldwide. The irrigation of fields with contaminated surface waters or their fertilization with sludge from wastewater treatment plants can lead to the introduction of Gd into the human food supply chain. Thus, this study focused on the potential toxicity of Gd on plants. For this purpose, we have studied the molecular effects of gadobutrol (a well-known MRI contrast agent) exposure on in vitro-grown Stevia rebaudiana. The effects of gadobutrol on plant morphology, on relevant plant metabolites such as chlorophylls, carotenoids, ascorbic acids (HPLC), minerals (ICP-OES), and on the generation of free radical species (MDA assay and EPR) were assessed. Exposures of 0.01, 0.05, 0.1, 1, and 3 mM gadobutrol were used. We found a correlation between the gadobutrol dose and the plant growth and concentration of metabolites. Above the 0.1. mM dose of gadobutrol, the toxic effects of Gd+3 ions became significant.
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Popova V, Petkova Z, Mazova N, Ivanova T, Petkova N, Stoyanova M, Stoyanova A, Ercisli S, Okcu Z, Skrovankova S, Mlcek J. Chemical Composition Assessment of Structural Parts (Seeds, Peel, Pulp) of Physalis alkekengi L. Fruits. Molecules 2022; 27:molecules27185787. [PMID: 36144521 PMCID: PMC9501157 DOI: 10.3390/molecules27185787] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/09/2022] [Revised: 09/01/2022] [Accepted: 09/02/2022] [Indexed: 11/16/2022] Open
Abstract
In recent years there has been an extensive search for nature-based products with functional potential. All structural parts of Physalis alkekengi (bladder cherry), including fruits, pulp, and less-explored parts, such as seeds and peel, can be considered sources of functional macro- and micronutrients, bioactive compounds, such as vitamins, minerals, polyphenols, and polyunsaturated fatty acids, and dietetic fiber. The chemical composition of all fruit structural parts (seeds, peel, and pulp) of two phenotypes of P. alkekengi were studied. The seeds were found to be a rich source of oil, yielding 14–17%, with abundant amounts of unsaturated fatty acids (over 88%) and tocopherols, or vitamin E (up to 5378 mg/kg dw; dry weight). The predominant fatty acid in the seed oils was linoleic acid, followed by oleic acid. The seeds contained most of the fruit’s protein (16–19% dw) and fiber (6–8% dw). The peel oil differed significantly from the seed oil in fatty acid and tocopherol composition. Seed cakes, the waste after oil extraction, contained arginine and aspartic acid as the main amino acids; valine, phenylalanine, threonine, and isoleucine were present in slightly higher amounts than the other essential amino acids. They were also rich in key minerals, such as K, Mg, Fe, and Zn. From the peel and pulp fractions were extracted fruit concretes, aromatic products with specific fragrance profiles, of which volatile compositions (GC-MS) were identified. The major volatiles in peel and pulp concretes were β-linalool, α-pinene, and γ-terpinene. The results from the investigation substantiated the potential of all the studied fruit structures as new sources of bioactive compounds that could be used as prospective sources in human and animal nutrition, while the aroma-active compounds in the concretes supported the plant’s potential in perfumery and cosmetics.
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Affiliation(s)
- Venelina Popova
- Department of Tobacco, Sugar, Vegetable and Essential Oils, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Zhana Petkova
- Department of Chemical Technology, Faculty of Chemistry, University of Plovdiv “Paisii Hilendarski”, 4000 Plovdiv, Bulgaria
| | - Nadezhda Mazova
- Department of Engineering Ecology, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Tanya Ivanova
- Department of Tobacco, Sugar, Vegetable and Essential Oils, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Nadezhda Petkova
- Department of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Magdalena Stoyanova
- Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Albena Stoyanova
- Department of Tobacco, Sugar, Vegetable and Essential Oils, University of Food Technologies, 4002 Plovdiv, Bulgaria
| | - Sezai Ercisli
- Department of Horticulture, Atatürk University, 25240 Erzurum, Turkey
| | - Zuhal Okcu
- Department of Gastronomy, Faculty of Tourism, Ataturk University, 25240 Erzurum, Turkey
| | - Sona Skrovankova
- Department of Food Analysis and Chemistry, Tomas Bata University in Zlin, 76001 Zlin, Czech Republic
- Correspondence: ; Tel.: +420-57603-1524
| | - Jiri Mlcek
- Department of Food Analysis and Chemistry, Tomas Bata University in Zlin, 76001 Zlin, Czech Republic
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Leopold LF, Coman C, Clapa D, Oprea I, Toma A, Iancu ȘD, Barbu-Tudoran L, Suciu M, Ciorîță A, Cadiș AI, Mureșan LE, Perhaița IM, Copolovici L, Copolovici DM, Copaciu F, Leopold N, Vodnar DC, Coman V. The effect of 100-200 nm ZnO and TiO 2 nanoparticles on the in vitro-grown soybean plants. Colloids Surf B Biointerfaces 2022; 216:112536. [PMID: 35567806 DOI: 10.1016/j.colsurfb.2022.112536] [Citation(s) in RCA: 25] [Impact Index Per Article: 8.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2022] [Revised: 04/29/2022] [Accepted: 05/01/2022] [Indexed: 01/22/2023]
Abstract
Engineered nanomaterials are increasingly used in everyday life applications and, in consequence, significant amounts are being released into the environment. From soil, water, and air they can reach the organelles of edible plants, potentially impacting the food chain and human health. The potential environmental and health impact of these nanoscale materials is of public concern. TiO2 and ZnO are among the most significant nanomaterials in terms of production amounts. Our study aimed at evaluating the effects of large-scale TiO2 (~100 nm) and ZnO (~200 nm) nanoparticles on soybean plants grown in vitro. The effect of different concentrations of nanoparticles (10, 100, 1000 mg/L) was evaluated regarding plant morphology and metabolic changes. ZnO nanoparticles showed higher toxicity compared to TiO2 in the experimental set-up. Overall, elevated levels of chlorophylls and proteins were observed, as well as increased concentrations of ascorbic and dehydroascorbic acids. Also, the decreasing stomatal conductance to water vapor and net CO2 assimilation rate show higher plant stress levels. In addition, ZnO nanoparticle treatments severely affected plant growth, while TEM analysis revealed ultrastructural changes in chloroplasts and rupture of leaf cell walls. By combining ICP-OES and TEM results, we were able to show that the nanoparticles were metabolized, and their internalization in the soybean plant tissues occurred in ionic forms. This behavior most likely is the main driving force of nanoparticle toxicity.
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Affiliation(s)
- Loredana F Leopold
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Cristina Coman
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Doina Clapa
- Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Ioana Oprea
- Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Alexandra Toma
- Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Ștefania D Iancu
- Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084 Cluj-Napoca, Romania.
| | - Lucian Barbu-Tudoran
- Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania.
| | - Maria Suciu
- Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania.
| | - Alexandra Ciorîță
- Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania.
| | - Adrian I Cadiș
- Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania.
| | - Laura Elena Mureșan
- Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania.
| | - Ioana Mihaela Perhaița
- Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania.
| | - Lucian Copolovici
- Institute for Research, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania; Faculty of Food Engineering, Tourism and Environmental Protection, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania.
| | - Dana M Copolovici
- Institute for Research, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania; Faculty of Food Engineering, Tourism and Environmental Protection, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania.
| | - Florina Copaciu
- Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Nicolae Leopold
- Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084 Cluj-Napoca, Romania.
| | - Dan C Vodnar
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
| | - Vasile Coman
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania.
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Micropropagation of Vaccinium corymbosum L.: An Alternative Procedure for the Production of Secondary Metabolites. HORTICULTURAE 2022. [DOI: 10.3390/horticulturae8060480] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/28/2022]
Abstract
In vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain compounds, this technology has enormous potential for the manufacture of natural bioactive chemicals. As a result, the goal of this study was to develop an efficient micropropagation system for biomass production and to investigate the accumulation of bioactive compounds from Vaccinium corymbosum L., Duke and Hortblue Petite cultivars. Two in vitro plant tissue culture systems were used for shoots production: a solid medium (5 g/L Plant agar) and liquid medium (Plantform bioreactor). The culture medium used was Woddy Plant Medium (WPM) supplemented with two growth regulators: 0.5 mg/L and 1 mg/L zeatina (Z) and 5 mg/L N6-(2-Isopentenyl) adenine (2iP). The content of phenolic compounds, carotenoids, and chlorophylls of the in vitro shoot extracts were examined via the HPLC-DAD-MS/MS technique. The results showed that cv. Hortblue Petite produced a higher amount of biomass compared with cv. Duke, on all variants of culture media in both systems (solid and liquid), while the shoots extract of the Duke variety in the liquid culture system (under all concentrations of growth regulators) had the highest content of total phenolic compounds (16,665.61 ± 424.93 μg/g). In the case of the lipophilic compounds analysed (chlorophylls and carotenoids), the solid medium reported the highest values, whereas media supplemented with 0.5 mg/L Z was proved to have the richest total content for both cultivars.
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Kim SH, Lee YC. Plant-Derived Nanoscale-Encapsulated Antioxidants for Oral and Topical Uses: A Brief Review. Int J Mol Sci 2022; 23:ijms23073638. [PMID: 35409001 PMCID: PMC8998173 DOI: 10.3390/ijms23073638] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/11/2022] [Revised: 03/23/2022] [Accepted: 03/24/2022] [Indexed: 02/04/2023] Open
Abstract
Several plant-based nanoscale-encapsulated antioxidant compounds (rutin, myricetin, β-carotene, fisetin, lycopene, quercetin, genkwanin, lutein, resveratrol, eucalyptol, kaempferol, glabridin, pinene, and whole-plant bio-active compounds) are briefly introduced in this paper, along with their characteristics. Antioxidants’ bioavailability has become one of the main research topics in bio-nanomedicine. Two low patient compliance drug delivery pathways (namely, the oral and topical delivery routes), are described in detail in this paper, for nanoscale colloidal systems and gel formulations. Both routes and/or formulations seek to improve bioavailability and maximize the drug agents’ efficiency. Some well-known compounds have been robustly studied, but many remain elusive. The objective of this review is to discuss recent studies and advantages of nanoscale formulations of plant-derived antioxidant compounds.
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Long JT, Fan HX, Zhou ZQ, Sun WY, Li QW, Wang Y, Ma M, Gao H, Zhi H. The major zeaxanthin dipalmitate derivatives from wolfberry. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH 2020; 22:746-753. [PMID: 31163996 DOI: 10.1080/10286020.2019.1621855] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/26/2019] [Revised: 05/16/2019] [Accepted: 05/17/2019] [Indexed: 06/09/2023]
Abstract
Zeaxanthin dipalmitate (3) and two zeaxanthin dipalmitate derivatives, including one new compound (1), were obtained from wolfberry [the fruit of Lycium barbarum L. (Solanaceae)]. Their structures were unambiguously elucidated by spectroscopic analyses. Compound 2 is isolated from the genus Lycium for the first time, and its 1D/2D NMR data are firstly reported. All the compounds belong to carotenoids which are a kind of major bioactive constituents in wolfberry and are also responsible for wolfberry's red color.
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Affiliation(s)
- Jia-Tang Long
- School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
| | - Hong-Xia Fan
- College of Traditional Chinese Medicine/Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China
| | - Zheng-Qun Zhou
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
| | - Wan-Yang Sun
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
| | - Qing-Wen Li
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
| | - Ying Wang
- Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
| | - Min Ma
- College of Traditional Chinese Medicine/Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China
| | - Hao Gao
- Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
| | - Hui Zhi
- School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
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Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2020; 2020:1736803. [PMID: 32655657 PMCID: PMC7327606 DOI: 10.1155/2020/1736803] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/11/2020] [Revised: 05/21/2020] [Accepted: 06/03/2020] [Indexed: 12/04/2022]
Abstract
Background Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein-c but, in the last decade, hepatotoxicity, associated with liver injuries in the next months after treatments' initiation, was reported. In this case, associated phytotherapy can be a solution. Purpose To investigate the antioxidant potential and response to free radicals, in the case of hyperlipidemic rats treated with atorvastatin. Sea buckthorn (Hippophae rhamnoides) and a grape extract (antioxivita) efficiency in the oxidative stress were investigated, also being ascertained the rats' organs cytoarchitecture. Methods Eighty-four hyperlipidemic Wistar rats were divided into seven groups and orally treated as follows: ATS, atorvastatin (20 mg/kg·bw); ATS + Hr, atorvastatin + H. rhamnoides; ATS + Aox, atorvastatin + grape extract; Hr, H. rhamnoides; and Aox, grape extract (both as 100 mg/kg·bw). HFD and Control received high fat diet and normal fodder only. After two and six months, respectively, rats were euthanized and the heart, liver, and kidneys were gathered. The tissue samples were prepared by homogenization of 0.5 g tissue, in ethanol, kept for 48 hours at 4°C–10°C and then filtered, in order to assess organs' cytoarchitecture and the TAC's values (by using cupric ion reducing antioxidant capacity (CUPRAC) assay). The test tubes were incubated, at room temperature, for 30 minutes, and then analyzed using a spectrophotometer at 450–650 nm. Results The statistics (ANOVA) revealed that sea buckthorn diminished notably (p < 0.001) the oxidative stress in the heart, liver, and kidney. After six months, the TAC's reduced levels for the heart were significant (p < 0.001) in ATS + Aox. In the case of histology, the liver's cytoarchitecture in ATS revealed abnormal cytoarchitecture. In ATS + Hr, ATS + Aox, Hr, and Aox, cell regeneration improved in different stages, especially for ATS + Hr and ATS + Aox, in comparison with HFD, which exhibited fat degeneration. Kidney's cytoarchitecture revealed cellular healing, especially in ATS + Hr and ATS + Aox.
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Ciesarová Z, Murkovic M, Cejpek K, Kreps F, Tobolková B, Koplík R, Belajová E, Kukurová K, Daško Ľ, Panovská Z, Revenco D, Burčová Z. Why is sea buckthorn (Hippophae rhamnoides L.) so exceptional? A review. Food Res Int 2020; 133:109170. [PMID: 32466930 DOI: 10.1016/j.foodres.2020.109170] [Citation(s) in RCA: 122] [Impact Index Per Article: 24.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/30/2019] [Revised: 03/09/2020] [Accepted: 03/15/2020] [Indexed: 01/23/2023]
Abstract
Sea buckthorn (Hippophae L.) is a valuable, multipurpose plant extensively grown in Asia, Europe and Canada. In order to use it in the best way for products of human nutrition, it is necessary to recognize its positive aspects and to eliminate the negative ones. The exceptional value of sea buckthorn can be seen in the presence of both lipophilic antioxidants (mainly carotenoids and tocopherols) and hydrophilic antioxidants (flavonoids, tannins, phenolic acids, ascorbic acid) in remarkably high quantities. Some of the main nutrients, especially lipids of advantageous fatty acid composition, contribute to nutritional benefits of sea buckthorn products for a consumer as well. This review article focuses, besides the above mentioned compounds and vitamins, also on other important components, such as sugars, sugar derivatives, fibre, organic acids, proteins, amino acids and mineral elements. The article also deals with the effects of sea buckthorn components on the course of non-enzymatic browning of food and in vivo glycation. In addition, sensory perception of sea buckthorn and its constituents from the consumers point of view is discussed.
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Affiliation(s)
- Zuzana Ciesarová
- NPPC National Agricultural and Food Centre, Food Research Institute, Priemyselná 4, 824 75 Bratislava, the Slovak Republic.
| | - Michael Murkovic
- Graz University of Technology, Faculty of Technical Chemistry, Chemical and Process Engineering and Biotechnology, Institute of Biochemistry, Petersgasse 12/II, 8010 Graz, Austria
| | - Karel Cejpek
- University of Chemistry and Technology, Prague, Faculty of Food and Biochemical Technology, Technická 5, 166 28 Praha 6, the Czech Republic
| | - František Kreps
- Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Radlinského 9, 812 37 Bratislava, the Slovak Republic
| | - Blanka Tobolková
- NPPC National Agricultural and Food Centre, Food Research Institute, Priemyselná 4, 824 75 Bratislava, the Slovak Republic
| | - Richard Koplík
- University of Chemistry and Technology, Prague, Faculty of Food and Biochemical Technology, Technická 5, 166 28 Praha 6, the Czech Republic
| | - Elena Belajová
- NPPC National Agricultural and Food Centre, Food Research Institute, Priemyselná 4, 824 75 Bratislava, the Slovak Republic
| | - Kristína Kukurová
- NPPC National Agricultural and Food Centre, Food Research Institute, Priemyselná 4, 824 75 Bratislava, the Slovak Republic
| | - Ľubomír Daško
- NPPC National Agricultural and Food Centre, Food Research Institute, Priemyselná 4, 824 75 Bratislava, the Slovak Republic
| | - Zdenka Panovská
- University of Chemistry and Technology, Prague, Faculty of Food and Biochemical Technology, Technická 5, 166 28 Praha 6, the Czech Republic
| | - Diomid Revenco
- University of Chemistry and Technology, Prague, Faculty of Food and Biochemical Technology, Technická 5, 166 28 Praha 6, the Czech Republic
| | - Zuzana Burčová
- Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Radlinského 9, 812 37 Bratislava, the Slovak Republic
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Giuffrida D, Cacciola F, Mapelli-Brahm P, Stinco CM, Dugo P, Oteri M, Mondello L, Meléndez-Martínez AJ. Free carotenoids and carotenoids esters composition in Spanish orange and mandarin juices from diverse varieties. Food Chem 2019; 300:125139. [DOI: 10.1016/j.foodchem.2019.125139] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/03/2018] [Revised: 06/19/2019] [Accepted: 07/05/2019] [Indexed: 10/26/2022]
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Szabo K, Dulf FV, Diaconeasa Z, Vodnar DC. Antimicrobial and antioxidant properties of tomato processing byproducts and their correlation with the biochemical composition. Lebensm Wiss Technol 2019. [DOI: 10.1016/j.lwt.2019.108558] [Citation(s) in RCA: 44] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/01/2022]
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Wang Y, Wang SL, Zhang JY, Song XN, Zhang ZY, Li JF, Li S. Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent. JOURNAL OF ETHNOPHARMACOLOGY 2018; 211:197-206. [PMID: 28964871 DOI: 10.1016/j.jep.2017.09.004] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/31/2016] [Revised: 07/04/2017] [Accepted: 09/07/2017] [Indexed: 06/07/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Physalis alkekengi L. var. franchetii (Solanaceae) has been widely used in Chinese folk medicine due to its wide distribution throughout the country, for the treatment of a wide range of diseases including heat and cold, sore throat, fever, fungal infection, inflammation, toothache, rheumatism, burn, analgesic, ulcer and urinary diseases. However, the effect of P. alkekengi var. franchetii on ulcer and Helicobacter pylori infection has not been reported to date. AIM OF THE STUDY This study was designed to investigate the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of ethyl acetate fraction of the crude aqueous methanolic extract from the aerial parts of the plant P. alkekengi L. var. franchetii in rodents. MATERIALS AND METHODS Acute toxicity of the crude extract of P. alkekengi L. var. franchetii (PAF) was evaluated in rats. The petroleum ether fraction (PEF), butanol fraction (BF), ethyl acetate fraction (EAF) and aqueous fraction (AF) of crude aqueous methanolic extract from PAF were screened for anti-inflammatory and anti-ulcer potential at doses of 100, 250 and 500mg/kg (p.o.), using carrageenin-induced hind paw edema and ethanol-induced gastric lesions test in rats. In vitro anti-Helicobacter pylori activity of EAF was assayed subsequently. In addition, three doses of EAF were evaluated for analgesic activity using hot plate and writhing tests, respectively. Finally, we performed a phytochemical analysis of EAF. RESULTS Four fractions of crude extract from PAF significantly reduced the paw volume in carrageenin-induced hind paw edema model at different doses (100, 250 and 500mg/kg, p.o.). The fraction EAF at a dose of 500mg/kg exhibited the highest (75.92%) (0.150 ± 0.045***, ***p < 0.001) anti-inflammatory potential, which is similar to indomethacin (***P < 0.001)（0.120 ± 0.014***, 80.74% inhibition of inflammation） at 5mg/kg. Pretreatment with EAF (500mg/kg, p.o.) significantly reduced the intensity of gastric mucosal damage and showed higher gastroprotective activity (90.6%) when compared to the standard drug famotidine (84.6%). In addition, EAF fraction also showed a moderate (P < 0.05) anti-Helicobacter pylori activity with a minimal inhibition concentration (MIC) of 500μg/ml. Furthermore, pain sensation was effectively inhibited at 500mg/kg, p.o. of EAF as manifested by an increase (p < 0.001) of latency time in hot plate from 30 to 90min and a decrease (p < 0.001) in count of writhing induced by acetic acid. By HPLC, we determined some steroid, terpenoid and flavonoids (four compounds): kaempferol, quercetin, Blumenol A and physalindicanols A, which were isolated from the ethyl acetate fraction and identified using 1H NMR and 13C NMR spectra analysis. CONCLUSION This study demonstrated the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of EAF of the crude extract from PAF thus justifying its traditional usage.
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Affiliation(s)
- Yong Wang
- Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 TongJiaXiang, Nanjing 210009, Jiangsu Province, PR China; Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.
| | - Sui Lou Wang
- Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 TongJiaXiang, Nanjing 210009, Jiangsu Province, PR China.
| | - Jiong Yi Zhang
- Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China
| | - Xiao Ning Song
- Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China
| | - Zhi Yong Zhang
- Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China
| | - Jing Feng Li
- Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China
| | - Song Li
- Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China
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Kranjc E, Albreht A, Vovk I, Glavnik V. High performance thin-layer chromatography–mass spectrometry enables reliable analysis of physalins in different plant parts of Physalis alkekengi L. J Chromatogr A 2017; 1526:137-150. [DOI: 10.1016/j.chroma.2017.09.070] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2017] [Revised: 09/27/2017] [Accepted: 09/28/2017] [Indexed: 01/08/2023]
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Pop OL, Dulf FV, Cuibus L, Castro-Giráldez M, Fito PJ, Vodnar DC, Coman C, Socaciu C, Suharoschi R. Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei. Int J Mol Sci 2017; 18:ijms18122513. [PMID: 29186761 PMCID: PMC5751116 DOI: 10.3390/ijms18122513] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/05/2017] [Revised: 11/20/2017] [Accepted: 11/21/2017] [Indexed: 01/17/2023] Open
Abstract
Probiotics are bacteria that can provide health benefits to consumers and are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heat treatment and with an in vitro gastrointestinal model. The characterization of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis), high-performance liquid chromatography photodiode array detection method (HPLC-PDA), gas chromatography coupled with mass spectrometry (GS-MS) and Cryo scanning electron microscopy (Cryo-SEM). During heat treatment, the entrapped probiotic cells proved high viability (>6 CFU log/g), even at temperatures above 50 °C. The rich in monounsaturated fatty acids sea buckthorn fraction improved the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15% higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this approach may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.
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Affiliation(s)
- Oana Lelia Pop
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
| | - Francisc Vasile Dulf
- Department of Environmental and Plant Protection, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania;
| | - Lucian Cuibus
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
| | - Marta Castro-Giráldez
- Instituto Universitario de Ingeniería de Alimentos para el Desarrollo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain; (M.C.-G.); (P.J.F.)
| | - Pedro J. Fito
- Instituto Universitario de Ingeniería de Alimentos para el Desarrollo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain; (M.C.-G.); (P.J.F.)
| | - Dan Cristian Vodnar
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
| | - Cristina Coman
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
| | - Carmen Socaciu
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
| | - Ramona Suharoschi
- Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania; (O.L.P.); (L.C.); (D.C.V.); (C.C.); (C.S.)
- Correspondence: ; Tel.: +40-730-630-252
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Separation and identification of fatty acid esters of algal carotenoid metabolites in the freshwater mussel Dreissena bugensis , by liquid chromatography with ultraviolet/visible wavelength and mass spectrometric detectors in series. J Chromatogr A 2017; 1513:93-106. [DOI: 10.1016/j.chroma.2017.07.021] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/09/2017] [Revised: 06/27/2017] [Accepted: 07/06/2017] [Indexed: 11/19/2022]
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Wen X, Hempel J, Schweiggert RM, Ni Y, Carle R. Carotenoids and Carotenoid Esters of Red and Yellow Physalis (Physalis alkekengi L. and P. pubescens L.) Fruits and Calyces. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017; 65:6140-6151. [PMID: 28696106 DOI: 10.1021/acs.jafc.7b02514] [Citation(s) in RCA: 30] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/24/2023]
Abstract
Carotenoid profiles of fruits and calyces of red (Physalis alkekengi L.) and yellow (P. pubescens L.) Physalis were characterized by HPLC-DAD-APCI-MSn. Altogether 69 carotenoids were detected in red Physalis, thereof, 45 were identified. In yellow Physalis, 40 carotenoids were detected and 33 were identified. Zeaxanthin esters with various fatty acids were found to be the most abundant carotenoids in red Physalis, accounting for 51-63% of total carotenoids, followed by β-cryptoxanthin esters (16-24%). In yellow Physalis, mainly free carotenoids such as lutein and β-carotene were found. Total carotenoid contents ranged between 19.8 and 21.6 mg/100 g fresh red Physalis fruits and 1.28-1.38 mg/100 g fresh yellow Physalis fruits, demonstrating that Physalis fruits are rich sources of dietary carotenoids. Yellow Physalis calyces contained only 153-306 μg carotenoids/g dry weight, while those of red Physalis contained substantially higher amounts (14.6-17.6 mg/g dry weight), thus possibly exhibiting great potential as a natural source for commercial zeaxanthin extraction.
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Affiliation(s)
- Xin Wen
- Institute of Food Science and Biotechnology, University of Hohenheim , 70599 Stuttgart, Germany
- College of Food Science and Nutritional Engineering, China Agricultural University , 100083 Beijing, China
- National Engineering Research Center for Fruit and Vegetable Processing , 100083 Beijing, China
- Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture , 100083 Beijing, China
| | - Judith Hempel
- Institute of Food Science and Biotechnology, University of Hohenheim , 70599 Stuttgart, Germany
| | - Ralf M Schweiggert
- Institute of Food Science and Biotechnology, University of Hohenheim , 70599 Stuttgart, Germany
| | - Yuanying Ni
- College of Food Science and Nutritional Engineering, China Agricultural University , 100083 Beijing, China
- National Engineering Research Center for Fruit and Vegetable Processing , 100083 Beijing, China
- Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture , 100083 Beijing, China
| | - Reinhold Carle
- Institute of Food Science and Biotechnology, University of Hohenheim , 70599 Stuttgart, Germany
- Biological Science Department, King Abdulaziz University , P.O. Box 80257, 21589 Jeddah, Saudi Arabia
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Mihalcea L, Turturică M, Ghinea IO, Barbu V, Ioniţă E, Cotârleț M, Stănciuc N. Encapsulation of carotenoids from sea buckthorn extracted by CO 2 supercritical fluids method within whey proteins isolates matrices. INNOV FOOD SCI EMERG 2017. [DOI: 10.1016/j.ifset.2017.06.008] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/04/2023]
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Burčová Z, Kreps F, Schmidt Š, Jablonský M, Ház A, Sládková A, Šurina I. Composition of fatty acids and tocopherols in peels, seeds and leaves of Sea buckthorn. ACTA CHIMICA SLOVACA 2017. [DOI: 10.1515/acs-2017-0005] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
Abstract
Abstract
Peels, seeds and leaves of the Sea buckthorn were extracted by methanol and chloroform and characterized in terms of the fatty acids (FA) and tocopherol composition. All morphological parts of Sea buckthorn contained these biologically active compounds useful in medicine, pharmacology, human nutrition and cosmetics. The highest amount of α-tocopherol was found in peels (1103 mg kg-1). The lowest content of α-tocopherol was found in leaves (659 mg kg-1). Delta-tocopherol was found in higher amount in peels 1757 mg kg-1. Seeds contained 95 mg kg-1 of delta-tocopherols. Gamma-tocopherol was found in seeds (459 mg kg-1), peels (188 mg kg-1) and in leaves (587 mg kg-1). β-tocopherol was present only in seeds (171 mg kg-1). Unsaturated fatty acids were dominant in all morphological parts of Sea buckthorn. The highest amounts of unsaturated fatty acids (92 rel. %) were determined in seeds. Dominant fatty acids of seeds were linoleic acid (37 %), α-linolenic acid (30 %) and vaccenic acid (20 %). Leaves were rich in α-linolenic acid (51 %). Dominant fatty acids of peels were oleic acid (16 %), palmitic acid (33 %) and palmitoleic acid (29 %).
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Affiliation(s)
- Zuzana Burčová
- Department of Food Science and Technology; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - František Kreps
- Department of Food Science and Technology; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - Štefan Schmidt
- Department of Food Science and Technology; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - Michal Jablonský
- Department of Wood, Pulp and Paper; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - Aleš Ház
- Department of Wood, Pulp and Paper; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - Alexandra Sládková
- Department of Food Science and Technology; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
| | - Igor Šurina
- Department of Wood, Pulp and Paper; Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava , Slovak Republic
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Kranjc E, Albreht A, Vovk I, Makuc D, Plavec J. Non-targeted chromatographic analyses of cuticular wax flavonoids from Physalis alkekengi L. J Chromatogr A 2016; 1437:95-106. [PMID: 26875116 DOI: 10.1016/j.chroma.2016.01.061] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/16/2015] [Revised: 01/26/2016] [Accepted: 01/26/2016] [Indexed: 01/23/2023]
Abstract
Since Chinese lantern (Physalis alkekengi L.) represents a rich source of various bioactive secondary metabolites, there is an urge for its detailed characterization. Non-polar flavonoid aglycones represent one of the few bioactive species found in plant's cuticular waxes. The separation of flavonoids is already extensively covered in the literature, but methods dedicated to separation and identification of methylated flavonoids are rather scarce. In the present study a non-targeted approach for the separation, isolation and identification of methylated flavonoids present in P. alkekengi L. var. franchetii cuticular waxes was established. A rapid and simple separation on HPTLC silica gel was developed for preliminary screening of flavonoids. Fast HPLC-UV-MS(n) and HPLC-UV methods using a C6-Phenyl and a C18 stationary phase were also developed, respectively. In both cases, the right combination of temperature and tetrahydrofuran, as a mobile phase modifier, were shown to be crucial for a baseline separation of all studied compounds. By employing a semi-preparative analog of the C18 column, a simultaneous isolation of pure unknown analytes was achieved. Using these developed methods in combination with NMR, four 3-O-methylated flavonols were detected and identified in P. alkekengi L. var. franchetii cuticular waxes: myricetin 3,7,3'-trimethyl ether, quercetin 3,7-dimethyl ether, myricetin 3,7,3',5'-tetramethyl ether and quercetin 3,7,3'-trimethyl ether. Moreover, the simple and fast isocratic HPLC-UV-MS(n) method (under 8min) should prove useful in quality control of P. alkekengi L. var. franchetii by enabling chromatographic fingerprinting of external methylated flavonols. Finally, a rationale for the mechanism of separation of these metabolites by HPLC is also given, which establishes a foundation for future development of chromatographic methods for methylated flavonols and related compounds.
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Affiliation(s)
- Eva Kranjc
- Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
| | - Alen Albreht
- Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
| | - Irena Vovk
- Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.
| | - Damjan Makuc
- EN-FIST Centre of Excellence, Trg Osvobodilne Fronte 13, 1000 Ljubljana, Slovenia; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
| | - Janez Plavec
- EN-FIST Centre of Excellence, Trg Osvobodilne Fronte 13, 1000 Ljubljana, Slovenia; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1001 Ljubljana, Slovenia
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Shivapriya S, Ilango K, Dubey G. Evaluation of antioxidant and neuroprotective effect of Hippophae rhamnoides (L.) on oxidative stress induced cytotoxicity in human neural cell line IMR32. Saudi J Biol Sci 2015; 22:645-50. [PMID: 26288571 PMCID: PMC4537860 DOI: 10.1016/j.sjbs.2015.04.011] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/26/2015] [Revised: 04/10/2015] [Accepted: 04/12/2015] [Indexed: 01/03/2023] Open
Abstract
AIM AND OBJECTIVE Hippophae rhamnoides is an edible, nutrient rich plant found in the northern regions of India. It belongs to the family Elaeagnaceae and is well known for its traditional pharmacological activities. The present study was aimed to investigate the antioxidant and neuroprotective activities of H. rhamnoides. METHODOLOGY The hydroalcoholic extract of H. rhamnoides was evaluated for free radical scavenging activity using DPPH, hydroxyl radical scavenging and ferric thiocyanate assays. In vitro neuroprotective activity was assessed on human neuroblastoma cell line-IMR32 against hydrogen peroxide (H2O2) induced cytotoxicity. The neuroprotective effect was determined by measuring the cell viability through tetrazolium dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reducing assay and propidium iodide (PI) staining. Also the intracellular reactive oxygen species (ROS) activity was assessed using dichloro-dihydro-fluorescein diacetate (DCFDA) assay by flowcytometer. RESULTS The results of the study demonstrated that H. rhamnoides extract possesses potential free radical scavenging activity. The IC50 value for DPPH and OH radical scavenging assay was 70.92 μg/ml and 0.463 mg/ml, also the extract was also found to have considerable level of lipid peroxidation activity. The neuroprotective effect of H. rhamnoides was confirmed by its cell viability enhancing capacity against hydrogen peroxide induced cell cytotoxicity. The extract acted on IMR32 cells in a dose dependent manner as observed through PI and MTT assays. The percentage intracellular ROS activity was reduced by 60-70% in treated cells compared to H2O2 control. CONCLUSION Thus the outcome of the study suggests that H. rhamnoides acts as a neuroprotectant against oxidative stress induced neurodegeneration.
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Affiliation(s)
- S. Shivapriya
- Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur, Chennai 603203, India
| | - K. Ilango
- Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur, Chennai 603203, India
| | - G.P. Dubey
- Interdisciplinary School of Indian System of Medicine, SRM University, Kattankulathur, Chennai 603203, India
- Department of Kriya Sharir, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, U.P 221005, India
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Kulshrestha A, Jarouliya U, Prasad GBKS, Flora SJS, Bisen PS. Arsenic-induced abnormalities in glucose metabolism: Biochemical basis and potential therapeutic and nutritional interventions. World J Transl Med 2014; 3:96-111. [DOI: 10.5528/wjtm.v3.i2.96] [Citation(s) in RCA: 24] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 05/06/2014] [Revised: 06/21/2014] [Accepted: 07/17/2014] [Indexed: 02/05/2023] Open
Abstract
Health hazards due to the consumption of heavy metals such as arsenic have become a worldwide problem. Metabolism of arsenic produces various intermediates which are more toxic and cause toxicity. Arsenic exposure results in impairment of glucose metabolism, insulin secretion in pancreatic β-cells, altered gene expressions and signal transduction, and affects insulin-stimulated glucose uptake in adipocytes or skeletal muscle cells. Arsenic toxicity causes abnormalities in glucose metabolism through an increase in oxidative stress. Arsenic interferes with the sulfhydryl groups and phosphate groups present in various enzymes involved in glucose metabolism including pyruvate dehydrogenase and α-ketoglutarate dehydrogenase, and contributes to their impairment. Arsenic inhibits glucose transporters present in the cell membrane, alters expression of genes involved in glucose metabolism, transcription factors and inflammatory cytokines which stimulate oxidative stress. Some theories suggest that arsenic exposure under diabetic conditions inhibits hyperglycemia. However, the exact mechanism behind the behavior of arsenic as an antagonist or synergist on glucose homeostasis and insulin secretion is not yet fully understood. The present review delineates the relationship between arsenic and the biochemical basis of its relationship to glucose metabolism. This review also addresses potential therapeutic and nutritional interventions for attenuating arsenic toxicity. Several other potential nutritional supplements are highlighted in the review that could be used to combat arsenic toxicity.
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Pop RM, Weesepoel Y, Socaciu C, Pintea A, Vincken JP, Gruppen H. Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties. Food Chem 2014; 147:1-9. [DOI: 10.1016/j.foodchem.2013.09.083] [Citation(s) in RCA: 90] [Impact Index Per Article: 8.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/18/2012] [Revised: 05/23/2013] [Accepted: 09/13/2013] [Indexed: 11/25/2022]
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Physalis alkekengi carotenoidic extract inhibitor of soybean lipoxygenase-1 activity. BIOMED RESEARCH INTERNATIONAL 2014; 2014:589168. [PMID: 24511537 PMCID: PMC3910512 DOI: 10.1155/2014/589168] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/30/2013] [Revised: 10/14/2013] [Accepted: 10/18/2013] [Indexed: 11/18/2022]
Abstract
The aim of this study was to evaluate the effect of the carotenoidic saponified extract of Physalis alkekengi sepals (PA) towards the lipoxygenase (LOX) oxidation of linoleic acid. Lipoxygenase activity in the presence of carotenoids, standard and from extract, was followed by its kinetic behaviour determining the changes in absorption at 234 nm. The standard carotenoids used were β-carotene (β-car), lutein (Lut), and zeaxanthin (Zea). The calculated enzymatic specific activity (ESA) after 600 s of reaction proves that PA carotenoidic extract has inhibitory effect on LOX oxidation of linoleic acid. A longer polyenic chain of carotenoid structure gives a higher ESA during the first reaction seconds. This situation is not available after 600 s of reaction and may be due to a destruction of this structure by cooxidation of carotenoids, besides the classical LOX reaction. The PA carotenoidic extract inhibiting the LOX-1 reaction can be considered a source of lipoxygenase inhibitors.
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Giuffrida D, Pintea A, Dugo P, Torre G, Pop RM, Mondello L. Determination of carotenoids and their esters in fruits of sea buckthorn (Hippophae rhamnoides L.) by HPLC-DAD-APCI-MS. PHYTOCHEMICAL ANALYSIS : PCA 2012; 23:267-273. [PMID: 22473853 DOI: 10.1002/pca.1353] [Citation(s) in RCA: 31] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/05/2011] [Revised: 07/03/2011] [Accepted: 07/06/2011] [Indexed: 05/31/2023]
Abstract
INTRODUCTION The berries of Hippophae rhamnoides Linnaeus have high nutritional and medicinal values and have been used for centuries as food both in Europe and Asia. The oleoresins represent a potential source of carotenoid esters and can be used as food additives, cosmetic ingredients or nutraceuticals. OBJECTIVE The objective of this study was to develop a HPLC-DAD-APCI-MS method, with both positive and negative ionisation modes, for the direct identification of the native carotenoid composition in fruits of Hippophae rhamnoides. MATERIALS AND METHODS Fruits of Hippophae rhamnoides, cv. Serbanesti and Victoria, were collected from an experimental field at the Fruit Research Station of Bacau, Romania. Samples were extracted using methanol:ethyl acetate:petroleum ether (1:1:1, v/v/v). The HPLC-DAD-APCI-MS analyses were carried out on a Shimadzu system using a YMC C₃₀-column and a gradient elution. RESULTS In total 22 compounds were detected, eight were free carotenoids, nine were xanthophylls monoesters and five were xanthophylls diesters. Differences were observed in the relative percentage composition of the identified components among the two cultivars investigated. Zeaxanthin-C16:0,C16:0 was the most abundant diester. The unsaturated palmitoleic acid was directly detected in its esterified form, in zeaxanthin-C16:0,C16:1, which is reported here for the first time. Although present in small amounts the unsaturated oleic, linoleic, linolenic, hexadecadienoic and hexadecatrienoic acids were detected in their esterified forms as lutein monoesters, the last two having been detected in Hippophae rhamnoides for the first time. CONCLUSION A novel (HPLC-DAD-APCI-MS) method was developed for the direct identification of the native carotenoid composition in fruits of Hippophae rhamnoides.
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Affiliation(s)
- Daniele Giuffrida
- Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM. FF. NN, Università di Messina, Contrada Papardo, Salita Sperone 31, 98166 Messina, Italy.
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Suryakumar G, Gupta A. Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.). JOURNAL OF ETHNOPHARMACOLOGY 2011; 138:268-78. [PMID: 21963559 DOI: 10.1016/j.jep.2011.09.024] [Citation(s) in RCA: 230] [Impact Index Per Article: 16.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/19/2011] [Revised: 09/15/2011] [Accepted: 09/16/2011] [Indexed: 05/10/2023]
Abstract
UNLABELLED ETHNOPHARMACOLOGICAL CONTEXT: This review explores the medicinal and therapeutic applications of Sea buckthorn (Hippophae rhamnoides L.) in curtailing different types of acute as well as chronic maladies. The plant is being used in different parts of the world for its nutritional and medicinal properties. MATERIALS AND METHODS Sea buckthorn based preparations have been extensively exploited in folklore treatment of slow digestion, stomach malfunctioning, cardiovascular problems, liver injury, tendon and ligament injuries, skin diseases and ulcers. In the recent years, medicinal and pharmacological activities of Sea buckthorn have been well investigated using various in vitro and in vivo models as well as limited clinical trials. RESULTS Sea buckthorn has been scientifically analyzed and many of its traditional uses have been established using several biochemical and pharmacological studies. Various pharmacological activities such as cytoprotective, anti-stress, immunomodulatory, hepatoprotective, radioprotective, anti-atherogenic, anti-tumor, anti-microbial and tissue regeneration have been reported. CONCLUSION It is clear that Sea buckthorn is an important plant because of its immense medicinal and therapeutic potential. However, several knowledge gaps identified in this paper would give impetus to new academic and R&D activities especially for the development of Sea buckthorn based herbal medicine and nutraceuticals.
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Affiliation(s)
- Geetha Suryakumar
- Department of Biochemistry, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110 054, India.
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Xu YJ, Kaur M, Dhillon RS, Tappia PS, Dhalla NS. Health benefits of sea buckthorn for the prevention of cardiovascular diseases. J Funct Foods 2011. [DOI: 10.1016/j.jff.2011.01.001] [Citation(s) in RCA: 40] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022] Open
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Andersson SC, Olsson ME, Johansson E, Rumpunen K. Carotenoids in sea buckthorn ( Hippophae rhamnoides L.) berries during ripening and use of pheophytin a as a maturity marker. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2009; 57:250-8. [PMID: 19125686 DOI: 10.1021/jf802599f] [Citation(s) in RCA: 67] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/20/2023]
Abstract
Four cultivars of sea buckthorn berries were analyzed for their carotenoid and chlorophyll contents during ripening in three consecutive years. The different carotenoids generally increased in concentration during ripening and comprised from 120 to 1425 microg/g of DW of total carotenoids (1.5-18.5 mg/100 g of FW) depending on cultivar, harvest time, and year. GLM analyses revealed the effect of cultivar to be considerably larger than that of year and harvest time. The content of pheophytin a, a chlorophyll a derivate, steadily decreased during berry ripening. Pheophytin a therefore acted as a marker of the degree of ripening of sea buckthorn berries and was used here to convert harvest date into an estimated ripening time.
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Affiliation(s)
- Staffan C Andersson
- Department of Horticulture, Swedish University of Agricultural Sciences, Alnarp, Sweden.
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Current awareness in phytochemical analysis. PHYTOCHEMICAL ANALYSIS : PCA 2006; 17:63-70. [PMID: 16454478 DOI: 10.1002/pca.880] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/06/2023]
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Pintea A, Diehl HA, Momeu C, Aberle L, Socaciu C. Incorporation of carotenoid esters into liposomes. Biophys Chem 2005; 118:7-14. [PMID: 16002203 DOI: 10.1016/j.bpc.2005.06.007] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/06/2005] [Revised: 06/10/2005] [Accepted: 06/10/2005] [Indexed: 11/23/2022]
Abstract
Carotenoid esters are investigated for their interaction with liposomal membranes and compared with their corresponding free (non-esterified) carotenoids. A monoester (beta-cryptoxanthin) and two diesters (zeaxanthin and lutein) were chosen. Egg yolk phosphatidylcholine liposomes served as the membrane model. We measured the sizes of the liposomes by photon correlation spectroscopy. The incorporation yields were determined spectrophotometrically. From liposomes simultaneously doped with the fluorescent dye Laurdan, fluidity changes of the liposomes were obtained. In summary, the results indicate that the carotenoid esters: (i) get incorporated, but at a lower yield than their corresponding free carotenoids, (ii) also increase the membrane rigidity as do the free carotenoids, and (iii) increase the liposome sizes significantly, but after extrusion through an 0.1 mum filter the sizes resemble with the exception of the liposomes incorporated with lutein diesters, they remain bigger indicating an elastic property due to two different accessible locations in the membrane.
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Affiliation(s)
- Adela Pintea
- Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, Str. Manastur 3, RO-400372 Cluj-Napoca, Romania
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