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World J Virol. Feb 12, 2014; 3(1): 1-10
Published online Feb 12, 2014. doi: 10.5501/wjv.v3.i1.1
Immunological-based assays for specific detection of shrimp viruses
Parin Chaivisuthangkura, Siwaporn Longyant, Paisarn Sithigorngul
Parin Chaivisuthangkura, Siwaporn Longyant, Paisarn Sithigorngul, Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Parin Chaivisuthangkura, Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok 10110, Thailand
Author contributions: Chaivisuthangkura P wrote the manuscript; Longyant S provided and edited the Figures; Sithigorngul P advised for manuscript improvement.
Correspondence to: Dr. Parin Chaivisuthangkura, Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand. parin@swu.ac.th
Telephone: +66-2-6495000-18511 Fax: +66-2-2600128
Received: June 25, 2013
Revised: October 25, 2013
Accepted: November 15, 2013
Published online: February 12, 2014
Core Tip

Core tip: Monoclonal antibodies (MAbs) specific to various shrimp viruses were generated. The MAbs can be used to detect viral infection in shrimp by immunological assays such as Western blotting, dot blotting, and immunohistochemistry. Some of the MAbs were used to developed immunochromatographic strip tests for specific detection of white spot syndrome virus, yellow head virus, infectious myonecrosis virus, and Penaeus monodon nucleopolyhedrovirus formerly known as monodon baculovirus. The strip test has the advantages of speed, as the result can be obtained within 15 min, and simplicity, as laboratory equipment and specialized skills are not required.