Published online Feb 12, 2018. doi: 10.5501/wjv.v7.i1.10
Peer-review started: October 27, 2017
First decision: November 23, 2017
Revised: December 6, 2017
Accepted: December 13, 2017
Article in press: December 13, 2017
Published online: February 12, 2018
To identify cell culture models supportive for Zika virus (ZIKV) replication.
Various human and non-human cell lines were infected with a defined amount of ZIKV Polynesia strain. Cells were analyzed 48 h post infection for the amount of intracellular and extracellular viral genomes and infectious viral particles by quantitative real-time PCR and virus titration assay. The extent of replication was monitored by immunofluorescence and western blot analysis by using Env and NS1 specific antibodies. Innate immunity was assayed by luciferase reporter assay and immunofluorescence analysis.
All investigated cell lines except CHO cells supported infection, replication and release of ZIKV. While in infected A549 and Vero cells a pronounced cytopathic effect was observed COS7, 293T and Huh7.5 cells were most resistant. Although the analyzed cell lines released comparable amounts of viral genomes to the supernatant significant differences were found for the number of infectious viral particles. The neuronal cell lines N29.1 and SH-SY5Y released 100 times less infectious viral particles than Vero-, A549- or 293T-cells. However there is no strict correlation between the amount of produced viral particles and the induction of an interferon response in the analyzed cell lines.
The investigated cell lines with their different tissue origins and diverging ZIKV susceptibility display a toolbox for ZIKV research.
Core tip: In this study ten different cell lines, human and non-human, from various tissues (e.g., hepatocytes, keratinocytes and neuronal cells) were tested upon their susceptibility to Zika virus (ZIKV) infection. Except CHO cells all cells supported ZIKV life cycle, but differed in parts strongly in the intracellular and released amount of infectious viral particles. Investigating the interferon response showed no clear correlation between high and low producer cell lines.