Published online Jun 9, 2015. doi: 10.5497/wjp.v4.i2.227
Peer-review started: October 13, 2014
First decision: December 26, 2014
Revised: January 9, 2015
Accepted: April 8, 2015
Article in press: April 9, 2015
Published online: June 9, 2015
AIM: To investigate the efficacy of fu-qi granule (FQG) on carbon tetrachloride (CCl4) induced liver fibrosis in rats and the underlying mechanisms.
METHODS: Sixty rats were randomly divided into six groups: normal control group, CCl4 induced liver fibrosis group, AnluoHuaxianWan group and three treatment groups of FQG. Treatment of rats with intraperitoneal injection of carbon tetrachloride solution at 0.3 mL per 100 g body weigh twice a week for 8 wk. The normal control group the rats were given the media (olive oil) at the same time. In the first 2 wk, rats were raised with feedstuff (80% corn meal, 20% lard, 0.5% cholesterol). Serum samples were collected for alanine transaminase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein assay and typical histopathological changes was observed in Hematoxylin-eosin staining sections. Smooth muscle alpha actin (α-SMA) was analyzed with immunohistochemistry. Mammalian target of rapamycin (mTOR) and hypoxia-inducible factor-1 (HIF-1α) expressions were detected by Western blotting. Tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and matrix metalloproteinases-9 (MMP-9) were measured with semi-quantitative reverse transcriptase-polymerase chain reaction.
RESULTS: FQG significantly reduced the serum levels of alanine transaminase, aspartate aminotransferase, alkaline phosphatase and increased the serum contents of albumin, total protein in rats with liver fibrosis. Moreover, FQG promoted extracellular matrix degradation by increasing MMP-9 and inhibiting TIMP-1 and α-SMA. mTOR and HIF-1α expression in liver significantly decreased in the rats treated with FQG.
CONCLUSION: The results indicated that FQG significantly reverse fibrosis induced by CCl4, which should be developed as a new and promising preparation for the prevention of liver fibrosis.
Core tip: Fu-qi granule (FQG) is traditional Chinese medicine preparation to prove the antifibrotic properties of traditional Chinese drug composed of six medicinal herbs in rats treated with carbon tetrachloride. we checked activities of liver enzymes, histopathological changes within the liver as well as the expression of mammalian target of rapamycin (mTOR) and hypoxia-inducible factor-1 and tissue inhibitor of matrix metalloproteinases-1 and matrix metalloproteinases-9. FQG can attenuate liver fibrosis induced by carbon tetrachloride via mTOR/p70S6K signal transduction pathway.