Mutations of the brpR and brpS genes affect biofilm formation in Staphylococcus aureus

Table 1 Bacterial strains and plasmids used in this study

Bacterial strain	Description	Ref.
E. coli
DH5	Transformation efficient E. coli strain	[16]
S. aureus
Newman	S. aureus clinical isolate	[14]
JE2	S. aureus USA300 MRSA strain	[15]
NE272	S. aureus JE2 brpS::mariner mutant	[15]
NE671	S. aureus JE2 brpR::mariner mutant	[15]
NE1787	S. aureus JE2 srtA::mariner mutant	[15]
RN4220	Transformation-efficient S. aureus strain	[17]
Newman brpR	S. aureus Newman brpR::mariner mutant	This study
Newman brpS	S. aureus Newman brpS::mariner mutant	This study
Newman srtA	S. aureus Newman srtA::mariner mutant	This study
Plasmids
pXB3-1	pALC2073 plasmid with the brpR gene inserted	This study
pALC2073	Cloning vector with Apr, Cmr, and Tcr genes, and a Tc-inducible promoter	[18]
pAMZ1-3	pALC2073 plasmid with the brpS gene inserted	This study

Table 2 Primers used in this study

Primer	Sequence
GEX-5XC	5’- CCTAGGAGATCTCTTTCTGTC -3’
GEX-5XB	5’- GTTAATTTTACTAAACTTAAG -3’
MW2284I	5’- GAGCAGGTACCATGATGAAACTCAATTTATTTATCAATGCAAAAG-3’
MW2284M	5’- CAGCGAATTCTCAATGGTGATGGTGATGTATTGATAATCGCTCCTTTATAGATTTTAAAA -3’
CidA1	5’- TGCAACGATACATGTTCCTATG -3’
CidA2	5’- CTACAACTAGGAATCATCATTGTG -3’
LrgA1	5’- GCATCAAAACCAGCACACTTT -3’
LrgA2	5’- GACTTCGCCTAACTTAACAGC -3’
SaFtsZ1	5’- GGTGTAGGTGGTGGCGGTAA -3’
SaFtsZ2	5’- TCATTGGCGTAGATTTGTC -3’
SrtA1	5’- TCGCTGGTGTGGTACTTATC -3’
SrtA2	5’- CAGGTGTTGCTGGTCCTGGA -3’