Dental pulp stem cells: Novel cell-based and cell-free therapy for peripheral nerve repair

Figure 1 Schematic diagram representing the steps of obtaining cell-free conditioned medium from dental pulp stem cells. The dental pulp stem cells (DPSCs) were plated onto culture vessels and grown in culture medium containing 10% fetal bovine serum. When adherent cells reach 80%-90% confluence, the medium was discarded and the cells washed with phosphate-buffered saline. Then, the DPSCs were incubated in α MEM serum-free medium and incubated for 72 h. The supernatant representing the conditioned medium was collected and centrifuged, filtered and concentrated.