Published online Feb 12, 2016. doi: 10.5318/wjo.v6.i1.1
Peer-review started: September 28, 2014
First decision: December 19, 2014
Revised: November 4, 2015
Accepted: November 23, 2015
Article in press: November 25, 2015
Published online: February 12, 2016
AIM: To explore the anticataractogenic potential of hesperidin, a flavanone, in galactose-induced cataractogenesis.
METHODS: In this study, cataract was induced by administering galactose enriched food in a set of rats. Effect of different dosages of hesperidin (25, 50 and 75 mg/kg body weight) were administered simultaneously with galactose in prevention of cataract was determined in another set. In both sets of animals, the levels of peroxidation, oxidants (NO and OH), antioxidants (enzymatic: Superoxide dismutase, catalase, glutathione S-transferase, GPx and non-enzymatic: Reduced glutathione, vitamin E), aldose reductase and sorbitol were determined in the eye lens. In addition, glucose and lipid peroxidation levels were also tested in serum. The quantitative changes in lens inducible nitric oxide synthase (iNOS) and its expression were also determined using Western blot and real-time polymerase chain reaction analyses.
RESULTS: Galactose enriched food produced cataract in both the eye lens as a sequel to elevated serum glucose. Simultaneous administration of hesperidin not only reduced serum glucose but also prevented cataract development, through reduced levels of reactive oxygen species (NO and OH) and iNOS expression as well as elevated enzymic and non-enzymic antioxidants were observed in the eye lens.
CONCLUSION: These results indicate the preventive effect of hesperidin against cataract in hyperglycemic rats.
Core tip: Hesperidin acts as an anticataractogenic agent in preventing development of cataract upon galactose induction in rats. At all the doses tested, hesperidin was able to prevent deleterious changes caused by galactose in eye lens.