Nitidine chloride may mediate its antitumor effects by targeting kinesin family member 20A in colorectal cancer cells

Figure 1 Diagram depicting the screening process used for analyzing kinesin family member 20A mRNA dataset. GEO: Gene Expression Omnibus; TCGA: The Cancer Genome Atlas; GTEx: Genotype-Tissue Expression; SRA: Sequence read archive; SMD: Standardized mean difference; KIF20A: Kinesin family member 20A.

Figure 2 Downregulation of kinesin family member 20A expression in colorectal cancer cells after nitidine chloride treatment. A: Kinesin family member 20A (KIF20A) expression in colorectal cancer cells after nitidine chloride (NC) treatment; B: Molecular docking diagram of NC with KIF20A. ^aP < 0.001. NC: Nitidine chloride; CTL: The control group (cells treated with 0.1% DMSO); KIF20A: Kinesin family member 20A.

Figure 3 High expression of kinesin family member 20A at single-cell, tissue, and mRNA levels in colorectal cancer. A: Kinesin family member 20A (KIF20A) expression in single-cell data; B: KIF20A expression in spatial transcriptomics data; C: KIF20A expression in Bulk RNA mRNA data. KIF20A: Kinesin family member 20A; AUC: Area under the curve.

Figure 4 High expression of kinesin family member 20A in colorectal cancer tissues based on immunohistochemical staining. A: Kinesin family member 20A (KIF20A) expression in non-colorectal cancer (CRC) tissues; B: KIF20A expression in CRC tissues.

Figure 5 Kinesin family member 20A is highly expressed in colorectal cancer tissues and correlates with gender, tumor size, and tumor grade I-III in clinical baseline data. KIF20A: Kinesin family member 20A. ^bP < 0.01.

Figure 6 Colorectal cancer -related cells are inhibited after kinesin family member 20A knockdown. CRISPR: Clustered regularly interspaced short palindromic repeats.

Figure 7 Nitidine chloride may affect colorectal cancer progression by targeting kinesin family member 20A through regulation of pathways such as mitosis. A: Venn diagram intersection analysis of highly expressed genes in colorectal cancer (CRC), genes positively correlated with kinesin family member 20A, and downregulated genes after nitidine chloride (NC) treatment of CRC cells; B: Biological function enrichment analysis results of intersection genes; C: Volcano plot of sequencing data after NC treatment of CRC cells. NC: Nitidine chloride; CRC: Colorectal cancer; BP: Biological process; CC: Cellular component; MF: Molecular function; KEGG: Kyoto Encyclopedia of Genes and Genomes.