Role of cell cycle-related gene SAC3 domain containing 1 as a potential target of nitidine chloride in hepatocellular carcinoma progression

Figure 1 Research flowchart. CEGs: Commonly expressed genes; DEGs: Differentially expressed genes; GO: Gene ontology; HCC: Hepatocellular carcinoma; KEGG: Kyoto Encyclopedia of Genes and Genomes; NC: Nitidine chloride; PDB: Protein Data Bank; RNA-seq: RNA sequencing; SAC3D1: SAC3 domain containing 1; TCGA: The Cancer Genome Atlas.

Figure 2 SAC3 domain containing 1 was a potential target of nitidine chloride. A: Volcano plot of differentially expressed genes after nitidine chloride (NC) treatment; B: Docking mode of NC and SAC3 domain containing 1. SAC3D1: SAC3 domain containing 1.

Figure 3 The enrichment analysis of the intersection genes between SAC3 domain containing 1 commonly expressed genes and the downregulated differentially expressed genes after nitidine chloride interaction. A: The Gene Ontology (GO) Biological Process; B: GO cell components; C: GO molecular functions; D: Kyoto Encyclopedia of Genes and Genomes enrichment pathways of 247 intersecting genes were mapped, respectively. ATP: Adenosine triphosphate; KEGG: Kyoto Encyclopedia of Genes and Genomes.

Figure 4 Correlation analysis. CCN: Cyclins; CDK: Cyclin-dependent kinases.

Figure 5 Impact of SAC3 domain containing 1 knockdown on cell proliferation, invasion and migration. A: SAC3 domain containing 1 (SAC3D1) knockdown efficiency detection (real-time reverse transcriptase-polymerase chain reaction); B: Knockdown of SAC3D1 significantly inhibited proliferation of hepatocellular carcinoma (HCC) cells; C: Knockdown of SAC3D1 dramatically decreased the invasion and migration of HCC cells. ^aP < 0.05, ^cP < 0.001, ^dP < 0.001. NC: Nitidine chloride; SAC3D1: SAC3 domain containing 1.

Figure 6 Impact of SAC3 domain containing 1 knockdown on cell cycle. A: Impact of negative control group on cell cycle; B: Impact of SAC3 domain containing 1 knockdown on the cell cycle of hepatocellular carcinoma cells; C: Bar graph of the ratio of cell cycle distribution at different periods. FL: Fluorescence; PI: Propidium iodide; SAC3D1: SAC3 domain containing 1.

Figure 7 Impact of SAC3 domain containing 1 knockdown on apoptosis in hepatocellular carcinoma cells. A: Impact of negative control group on apoptosis of hepatocellular carcinoma (HCC) cells; B: Impact of SAC3 domain containing 1 knockdown on apoptosis in HCC cells; C: Bar graph of apoptosis rates at different stages. FL: Fluorescence; Q1: Necrotic cells; Q2: Late apoptotic cells; Q3: Early; Q4: Live cells; SAC3D1: SAC3 domain containing 1; 7-AAD: 7-aminoactinomycin D.