Observational Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Pathophysiol. Feb 15, 2018; 9(1): 18-27
Published online Feb 15, 2018. doi: 10.4291/wjgp.v9.i1.18
Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
Caroline Linninge, Bodil Roth, Charlotte Erlanson-Albertsson, Göran Molin, Ervin Toth, Bodil Ohlsson
Caroline Linninge, Göran Molin, Department of Food Technology, Engineering and Nutrition, Lund University, Lund S-223 63, Sweden
Bodil Roth, Bodil Ohlsson, Department of Internal Medicine, Skåne University Hospital, Lund University, Malmö S-205 02, Sweden
Charlotte Erlanson-Albertsson, Appetite Regulation Unit, Department of Experimental Medical Science, Lund University, Lund S-222 41, Sweden
Ervin Toth, Department of Gastroenterology, Skåne University Hospital, Lund University, Malmö S-205 02, Sweden
Author contributions: Roth B, Erlanson-Albertsson C, Molin G, Toth E, and Ohlsson B designed the study; Toth E was responsible for the endoscopic examinations and biopsy sampling; Linninge C and Roth B performed the microbial examinations; Ohlsson B wrote the initial draft of the manuscript; all authors contributed to the intellectual criticism of the manuscript and accepted the final version.
Supported by Development Foundation of Region Skåne (BO), No. F2014/354.
Institutional review board statement: The study was reviewed and approved by the Ethics Review Board of Lund University (2013/903).
Informed consent statement: The subjects provided written, informed consent prior to study enrollment.
Conflict-of-interest statement: The authors have no conflicts of interests to declare.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at bodil.ohlsson@med.lu.se. Consent from participants was not obtained for data sharing, but the presented data are anonymized and risk of identification is low.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Bodil Ohlsson, MD, PhD, Professor, Department of Internal Medicine, Skåne University Hospital, Lund University, Jan Waldenströms Street 15, Malmö S-205 02, Sweden. bodil.ohlsson@med.lu.se
Telephone: +46-40-331000 Fax: +46-40-336208
Received: August 30, 2017
Peer-review started: September 5, 2017
First decision: October 12, 2017
Revised: November 10, 2017
Accepted: December 6, 2017
Article in press: December 6, 2017
Published online: February 15, 2018
Core Tip

Core tip: Colon mucosa biopsies were collected from consecutive patients (n = 51) at the time of elective colonoscopy. Patients were grouped into patients with diverticular disease (DD) (n = 16) and controls without any diverticula (n = 35). The amount of Enterobacteriaceae and bacterial diversity were analyzed. Patients with DD had significantly higher levels of Enterobacteriaceae than controls (P = 0.043). Bacterial diversity did not differ between groups. All but 8 patients exhibited some kind of gastrointestinal symptoms, and 22 patients (43.1%) fulfilled the criteria for irritable bowel syndrome, without difference between groups (P = 0.212). Demography, socioeconomic status, lifestyle habits, inflammatory parameters, or gastrointestinal symptoms did not affect the gut microbiota examined.