Elsobky S, Crane AM, Margolis M, Carreon TA, Bhattacharya SK. Review of application of mass spectrometry for analyses of anterior eye proteome. World J Biol Chem 2014; 5(2): 106-114 [PMID: 24921002 DOI: 10.4331/wjbc.v5.i2.106]
Corresponding Author of This Article
Sanjoy K Bhattacharya, PhD, Bascom Palmer Eye Institute, University of Miami, 1638 NW 10th Avenue, Suite 707A, Miami, FL 33136, United States. sbhattacharya@med.miami.edu
Research Domain of This Article
Ophthalmology
Article-Type of This Article
Review
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Biol Chem. May 26, 2014; 5(2): 106-114 Published online May 26, 2014. doi: 10.4331/wjbc.v5.i2.106
Review of application of mass spectrometry for analyses of anterior eye proteome
Sherif Elsobky, Ashley M Crane, Michael Margolis, Teresia A Carreon, Sanjoy K Bhattacharya
Sherif Elsobky, Ashley M Crane, Michael Margolis, Teresia A Carreon, Sanjoy K Bhattacharya, Bascom Palmer Eye Institute, University of Miami, Miami, FL 33136, United States
Sherif Elsobky, Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom
Teresia A Carreon, Sanjoy K Bhattacharya, Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL 33136, United States
Author contributions: Elsobky S and Crane AM wrote a the first draft of review of a substantial portion of the manuscript and also incorporating changes during the course of review; Margolis M, Carreon TA and reviewed some sections of the manuscript; Bhattacharya SK provided overall architecture planning, defining boundary and writing sections of the manuscript.
Correspondence to: Sanjoy K Bhattacharya, PhD, Bascom Palmer Eye Institute, University of Miami, 1638 NW 10th Avenue, Suite 707A, Miami, FL 33136, United States. sbhattacharya@med.miami.edu
Telephone: +1-305-4824103 Fax: +1-305-3266547
Received: November 19, 2013 Revised: January 16, 2014 Accepted: March 3, 2014 Published online: May 26, 2014
Core Tip
Core tip: Mass spectrometric based proteomics has been an indispensable tool for molecular and cellular biology. The ability of mass spectrometry to identify and precisely quantify thousands of proteins from complex samples has contributed greatly to biology and medicine. Through this we have studied protein-protein interactions via affinity-based isolations on a small and proteome-wide scale, the mapping of numerous organelles, and the generation of quantitative protein profiles from diverse species. The anterior segment of the eye is one of the most complicated parts of the human body with over 5000 proteins identified. Proteomic analyses of different parts of the eye, in particular the anterior eye structures, involve high throughput methods that help identify proteins and their posttranslational modifications. In this article we review the current state of advancement in the identification of anterior chamber proteins. We will present our findings in the following order: cornea, aqueous humor, trabecular meshwork, ciliary body, iris and lens.
