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World J Biol Chem. May 26, 2014; 5(2): 224-230
Published online May 26, 2014. doi: 10.4331/wjbc.v5.i2.224
Extracellular O-linked β-N-acetylglucosamine: Its biology and relationship to human disease
Mitsutaka Ogawa, Koichi Furukawa, Tetsuya Okajima
Mitsutaka Ogawa, Koichi Furukawa, Tetsuya Okajima, Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466-0065, Japan
Mitsutaka Ogawa, Department of Bioscience, Nagahama Institute of Bio-Science and Technology, Shiga 526-0829, Japan
Author contributions: Ogawa M, Furukawa K and Okajima T contributed to the writing of the manuscript.
Supported by In part by a grant-in-aid for Challenging Exploratory Research (to Okajima T) from the Japan Society for the Promotion of Science; and Scientific Research on Innovative Areas (to Okajima T) from the Ministry of Education, Culture, Sports, Science and Technology; and a grant from the Takeda Foundation (to Okajima T)
Correspondence to: Tetsuya Okajima, MD, PhD, Associate Professor, Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065, Japan. tokajima@med.nagoya-u.ac.jp
Telephone: +81-52-7442068 Fax: +81-52-7442069
Received: November 12, 2013
Revised: January 20, 2014
Accepted: April 9, 2014
Published online: May 26, 2014
Processing time: 210 Days and 14.8 Hours
Abstract

The O-linked β-N-acetylglucosamine (O-GlcNAc)ylation of cytoplasmic and nuclear proteins regulates basic cellular functions and is involved in the etiology of neurodegeneration and diabetes. Intracellular O-GlcNAcylation is catalyzed by a single O-GlcNAc transferase, O-GlcNAc transferase (OGT). Recently, an atypical O-GlcNAc transferase, extracellular O-linked β-N-acetylglucosamine (EOGT), which is responsible for the modification of extracellular O-GlcNAc, was identified. Although both OGT and EOGT are regulated through the common hexosamine biosynthesis pathway, EOGT localizes to the lumen of the endoplasmic reticulum and transfers GlcNAc to epidermal growth factor-like domains in an OGT-independent manner. In Drosophila, loss of Eogt gives phenotypes similar to those caused by defects in the apical extracellular matrix. Dumpy, a membrane-anchored apical extracellular matrix protein, was identified as a major O-GlcNAcylated protein, and EOGT mediates Dumpy-dependent cell adhesion. In mammals, extracellular O-GlcNAc was detected on extracellular proteins including heparan sulfate proteoglycan 2, Nell1, laminin subunit alpha-5, Pamr1, and transmembrane proteins, including Notch receptors. Although the physiological function of O-GlcNAc in mammals has not yet been elucidated, exome sequencing identified homozygous EOGT mutations in patients with Adams-Oliver syndrome, a rare congenital disorder characterized by aplasia cutis congenita and terminal transverse limb defects. This review summarizes the current knowledge of extracellular O-GlcNAc and its implications in the pathological processes in Adams-Oliver syndrome.

Keywords: Extracellular O-linked β-N-acetylglucosamine; Notch; Adams-Oliver syndrome

Core tip: The O-linked β-N-acetylglucosamine (O-GlcNAc) on extracellular protein domains is the most recently identified O-glycosylation of epidermal growth factor repeat-containing proteins such as Notch receptors. This O-GlcNAc modification occurs in the secretory pathway by an endoplasmic reticulum-resident O-GlcNAc transferase, extracellular O-linked β-N-acetylglucosamine (EOGT). In Drosophila, Dumpy, a membrane-tethered cuticle protein, was identified as a major O-GlcNAcylated protein that mediates the interaction between epithelial cells and the extracellular matrix. In mammals, extracellular O-GlcNAc was detected on Hspg2, Nell1, Lama5, Pamr1, and Notch receptors, although the physiological function of O-GlcNAc in mammals has not yet been elucidated. However, the recent finding that EOGT is a causative gene for Adams-Oliver syndrome provided important insights into the significance of extracellular O-GlcNAc in mammals. This review summarizes the current knowledge of extracellular O-GlcNAc and its implications in the pathological processes in Adams-Oliver syndrome.