Role of “reduced-size” liver/bowel grafts in the “abdominal wall transplantation” era

Abstract

The evolution of multi-visceral and isolated intestinal transplant techniques over the last 3 decades has highlighted the technical challenges related to the closure of the abdomen at the end of the procedure. Two key factors that contribute to this challenge include: (1) Volume/edema of donor graft; and (2) loss of abdominal domain in the recipient. Not being able to close the abdominal wall leads to a variety of complications and morbidity that range from complex ventral hernias to bowel perforation. At the end of the 90’s this challenge was overcome by graft reduction during the donor operation or bench table procedure (especially reducing liver and small intestine), as well as techniques to increase the volume of abdominal cavity by pre-operative expansion devices. Recent reports from a few groups have demonstrated the ability of transplanting a full-thickness, vascularized abdominal wall from the same donor. Thus, a spectrum of techniques have co-evolved with multi-visceral and intestinal transplantation, ranging from graft reduction to enlarging the volume of the abdominal cavity. None of these techniques are free from complications, however in large-volume centers the combinations of both (graft reduction and abdominal widening, sometimes used in the same patient) could decrease the adverse events related to recipient’s closure, allowing a faster recovery. The quest for a solution to this unique challenge has led to the proposal and implementation of innovative solutions to enlarge the abdominal cavity.

Key Words: Abdominal wall transplant, Reduced-size graft, Combined liver-bowel transplantation

Core tip: Matching donors with recipients to perform liver-bowel transplantation is a challenging task, especially in front of pediatric candidates due to the shortage of suitable donors. Historically, the issue was overcome reducing the size of liver and bowel during donation in order to implant the combined graft in the small abdominal cavity of the recipient. Due to the presence of complications, the procedure has been improved by enlarging the abdominal cavity of the recipients, initially through conventional techniques used in hernia repair or trauma surgery and later by transplanting the donor abdominal wall into the recipient. Results are encouraging but limited to high experienced centers.

INTRODUCTION

Experience has shown that intestinal and multi-visceral transplantation (ITx) is a feasible and potentially life-saving procedure. Donor-recipient size discrepancies have however been the Achilles heel, limiting the pool of donor organs especially for pediatric recipients due to donor-to-recipient body weight ratio, ideally between 1.1 and 0.76[1] , and size mismatching makes primary closure of the recipient abdominal wall one of the important technical challenges related to intestinal transplantation, mainly due to two factors: loss of abdominal domain because of sepsis, enteric-cutaneous fistulas or multiple surgeries of the recipient[2]; and volume or post-reperfusion intestinal edema of the graft[3]. Achieving tension-free closure after bowel transplant is of utmost importance to avoid post-operative abdominal compartment syndrome, risking ischemia and necrosis of the graft[4].

Different options have been reported in literature when a fascia closure is impossible, in the case of a donor-recipient size mismatch that has been undertaken due to unavailability of smaller donors.

The two main approaches focus on (1) Volume reduction of the graft[5]; or (2) an enlargement of the recipient abdominal domain[6]. The first approach includes an anatomical reduction of the graft that mainly applies for pediatric transplantation to prevent high waitlist mortality rates, while the second approach focuses on techniques to enlarge the abdominal domain ,mainly used in > 18 years population.

Pre-transplant mortality has gradually decreased for pediatric candidates in United States (less than 3 per 100 waitlist years, while for adult candidates is at 22.1 per 100 waitlist years), but notably it is still higher for intestine-liver transplant candidates[7], especially represented by the pediatric population: The need of total parenteral nutrition puts children at risk for developing liver disease and subsequently life-threatening complications[8].

Since the 90’s, the conventional transplant approach has utilized small size donors. But given the shortage of donors that fulfill the ideal characteristics, transplant centers have been increasingly accepting organs with considerable graft mismatch. Reducing the size of transplanted organs, with a reduced-size composite liver-intestinal allograft using split techniques[9], has resulted in utilization of organs from donors up to five times larger than recipients[10].

The development of reduced-size isolated bowel grafts has improved the limited availability of donors for candidates weighing less than 10 kg due to the possibility to overcome donor-recipient size mismatches[11] greater than to 10:1 (body weight).

An alternate method to solve the issue of size mismatch involves abdominal wall reconstruction, enabling substantial expansion of the recipient’s abdominal domain, especially when more organs (like liver-bowel) are to be transplanted[12]. However, this is challenging since most recipients are poor candidates for plastic surgery techniques such as tissue advancement or flap closure of the defect because of many previous surgeries.

Few techniques of abdominal wall reconstruction have been reported, many of them already used in difficult abdominal wall hernia repair or trauma surgery. Staged closure of the abdomen has been described by the Birmingham (United Kingdom) group[13], reporting on 23 combined liver and bowel transplants closed using a Silastic^® sheet together with a vacuum occlusive dressing.

The skin of the abdominal wall is often more pliable than the underlying tissue, and closure is possible sometimes with the help of tissue expanders[14,15]: Accordingly, twenty cases of inflatable tissue expanders in ITx candidates were reported in international literature. Localization of tissue expanders were: Subcutaneously in 13; intraperitoneally in 4; placed retromuscularly and 1 intraperitoneally; 1 patient had biplanar tissue expander (intraperitoneally placed and extending retromuscularly) and in 1 localization was unreported.

Alternatively, common used techniques include absorbable mesh[16]: Five pediatric liver and intestinal living-donor transplant recipients were treated by Chicago group initially through an absorbable Polygalactin mesh and later , once a granulated tissue was present, by a split-thickness skin graft. Sometimes the use of non-absorbable mesh[17] has also been reported: a prosthetic mesh alone was used in three patients from Bologna series to perform abdominal reconstruction , only in one case followed by a myocutaneous flap.

Apart from traditional reconstructive techniques, alternative methods include bioengineered skin equivalent[18], a-cellular dermal matrix[19,20], frozen human fibroblast-derived dermis[21], non-vascularized rectus muscle fascia[22,23], and vascularized “split-thickness”[24] or “full-thickness” skin grafts[25-31], either with classical[25], microsurgical[32] or remote revascularization technique[33]. These techniques are summarized in Table 1.

Table 1 Techniques of abdominal wall closure after intestinal and multi-visceral transplantation.

Ref.	Children/adults with difficult closure	Techniques used for closure	Post-ITx complications related to closure
Nery et al[5], 1998	N.a./n.a. tot = 11 (+ 5 graft reduction/modification)	4 silastic or PTFE mesh	5 incomplete closure
		2 skin flap
		1 myocutaneous flap
		3 mesh + graft reduction
		1 skin flap + graft reduction
Alexandrides et al[4], 2000	9/6	7 goretex mesh	None
		4 myocutaneous flap
		3 silastic mesh
		1 abdominal expander
Levi et al[25], 2003	2/6	8 full-thickness wall graft	2 wall infarction
Charles et al[21], 2004	0/1	1 fibroblast-derived dermis	None
Drosou et al[18], 2005	0/4	4 bioengineered skin equivalent	None
Asham et al[19], 2006	0/1	1 acellular dermal matrix	None
Carlsen et al[2], 2007	8/6	7 goretex mesh	6 incisional hernia
		4 (+ 2) split-thickness skin graft
		2 (+ 2) skin flap
		1 (+ 1) fascia
Zanfi et al[3], 2008	0/13 (+ 2 graft reduction)	5 skin closure	6 incisional hernia
		1 staged closure	4 mesh infection
		4 prosthetic mesh	2 fistulas
		3 full-thickness wall graft	1 abdominal compartments
Gondolesi et al[22], 2009	10/6	16 non-vascularized rectus fascia	7 wall infections
Grevious et al[16], 2009	5/0	5 staged closure (meshà split-thickness skin graft)	1 fistula
Sheth et al[13], 2012	23/0	23 staged closure	2 abdominal compartment s.
Mangus et al[20], 2012	12/25	30 acellular dermal allograft	1 dehiscence
		7 mesh or donor fascia	5 incisional hernia
			2 fistulas
Vianna et al, 2013 (unpublished results)	0/1	1 full-thickness wall graft	N.a.
Weiner et al[15],2014	1/0	1 bi-planar tissue expander	None
Vaidya et al, 2015 (in Chennai) (unpublished results)	1 n.a.	1 full-thickness wall graft	N.a.
Haveman et al[35], 2016	0/1	1 full-thickness wall graft	None
Giele et al[24], 2016	0/19	17 full-thickness wall graft	3 wound infection
		1 partial-thickness vascularized graft 1 partial-Thickness nonvascularized graft

ITx: Intestinal and multi-visceral transplantation; PTEE: Partial-thickness nonvascularized graft.

The use of either vascularized “partial” (rectus fascia) or “full-thickness” abdominal wall insensate[34] grafts (obtained from the same donor as the intestinal organs) has been successfully done in both, adult[35] as well as pediatric population[25].

The vascularized donor abdominal wall may have an immunological impact as well[36], and it has been proposed as a “sentinel” graft[37-41]. An allograft skin rash may represent a rejection phenomenon occurring earlier than the bowel manifestations, allowing to minimize therapy because treatment of abdominal wall rejection (very often steroid-responsive) may prevent intestinal rejection, which is a much more difficult issue to handle pharmacologically.

It has been hypothesized that the combined skin-intestine allograft from the same donor could present diagnostic and therapeutic advantages to the patient and clinician. Furthermore it has also reported the benefit of the skin, from the vascularized abdominal wall, being used to detect graft versus host disease in recipients of a combined abdominal wall-bowel graft by identifying a body rash in the recipient that spares the skin of the abdominal wall graft[42,43].

DONOR PROCUREMENT IN CASE OF SIZE MISMATCH

Procurement strategies for combined multi-organ and composite tissues for transplantation[44] continue to evolve, from the initial reports back in the early 90’s. In case of donor-recipient size mismatch[5], the surgeon could reduce the graft or conversely retrieve an abdominal wall during donor operation.

Splitting both liver (left lateral segment represented by segments II and III) and intestine (ileum) during a combined transplantation, with resulting Roux-en-Y loop biliary reconstruction in the recipient, was first reported by Xenos et al[45] in 1999.

Another way to reduce the liver-bowel graft during the harvest was described by Reyes et al[9] isolating the intestine and removing it en-block with the left lateral liver segment (segment II and III, previously splitted in situ): Eliminating the need of biliary reconstruction reduces most technical complications and avoids the use of the bowel for bilio-digestive anastomosis.

A similar advantage was reported by de Ville de Goyet et al[10], where during the bench table surgery the liver was reduced, using an approach that leaves the liver hilum untouched.

Isolated intestinal grafts could be size-modified: Fifteen small bowels were successfully reduced by Delrivière et al[11] obtaining a one meter ileal graft vascularized by the superior mesenteric artery and vein. Later, technical modifications allowed the use of two grafts from a single donor, represented by part of ileum and part of jejunum.

These techniques are summarized in Figure 1.

Figure 1 Historical techniques of reduced-size bowel and liver-bowel grafts before intestinal and multi-visceral transplantation.

Two popular procedures have been reported in order to harvest an abdominal wall: In the original Miami technique[25] the vessels of the wall graft were represented by donor femoral and iliac vessels, together with a small patch of aorta and inferior vena cava used to implant them into the recipient’s common iliac artery and vein. A modified microsurgical procedure was later reported by Cipriani et al from Bologna[32], collecting only the donor epigastric vessels with the abdominal wall, so sparing donor femoral-iliac vascular axes by direct anastomosis of the inferior donor-recipient epigastric vessels.

Both the procedures (size reduction and abdominal wall retrieval) are time-consuming in both donor and recipient operations but it is worthwhile to notice that, to date, there have been only insensate abdominal wall graft retrievals without nerve coaptation, a factor that may further impact procurement time if added in the future[46].

REDUCED SIZE LIVER-BOWEL CADAVERIC TRANSPLANTATION

The “golden age” of the reduced size techniques was practiced till the back end of the 90’s. In 1998 Reyes et al[9] reported the cases of a 3-year-old boy with hepatic-intestinal failure and a 63-year-old man with a central hepatoma and hepatitis C cirrhosis, both transplanted using the same adult cadaveric donor. The donor left lateral hepatic segment (segment II and III) in continuity with the small intestine was implanted into the child, using a modified in situ split technique where biliary reconstruction is unnecessary, while the right side of the donor liver was transplanted into the man. The pediatric recipient was later re-transplanted due to a liver damage related to a native pancreatic fistula, while the adult patient died for rupture of pseudo-aneurysm related to infection of the arterial graft.

In 1999 Xenos et al[45] described the use in a child of split liver (left lateral segment represented by segment II and III) and partial intestine (ileum) from a cadaveric donor during a combined transplantation: The right side went to an adult discharged home without complications. The pediatric recipient underwent a Roux-en-Y loop biliary reconstruction: Later he died for intestinal perforation plus severe rejection.

In 2000, de Ville de Goyet et al[10] transplanted two children, weighing 7.6 and 9.8 kg respectively, with a composite graft procured from donors weighing 35 kg (almost five times larger): Both went home on full enteral feeds. The composite graft was obtained during bench table surgery (leaving the hepatic hilum untouched) and was represented by liver segment II and III and whole small bowel, including duodenum and pancreas head. Also in this case there was no need of biliary reconstruction due to the preservation of the donor duodenum in continuity with the combined graft.

ABDOMINAL WALL TRANSPLANTATION-TECHNIQUES AND RESULTS

At the beginning of the new millennium, a rather innovative method to overcome the donor-recipient size-mismatching was hypothesized and VCA (vascularized composite allograft) was first reported by Levi et al[25] in 2003 in the form of abdominal wall transplantation: Their idea was to cover at the end of an ITx the resulting abdominal wall defect with both donor rectus abdominis muscles plus fascia, subcutaneous tissue and skin. The Miami group transplanted the wall graft like a kidney allograft, using as a blood supply the donor inferior epigastric vessels (left in continuity with the femoral and iliac vessels), and implanting them into the recipient’s common iliac artery and vein. The procedure time was about 2 h and this full thickness, vascularized, myocutaneous free flap was finally rotated and positioned according to location of the abdominal wall defect. Doppler ultrasound was used to monitor the blood flow.

The procedure was later modified by the Bologna group[32], using a microsurgical technique with a Zeiss microscope (Oberkochen; Germany): The donor epigastric pedicles were anastomosed end-to-end with the recipient epigastric vessels with no need to collect the donor femoral and iliac vessels. The operative time was similar to the one reported by Miami group.

Giele et al[33] from Oxford (United Kingdom) faced a different issue related to abdominal wall transplantation: The storage and subsequent ischemia-reperfusion injury of the wall graft during > 5 h ITx procedures. The ischemic time was minimized by two teams working at the same time on the recipient, one performing the intestinal transplant and the other re-vascularizing the abdominal wall remotely on the recipient forearm vessels. The procedure time lasted 50 min (30-60 min). Later the wall graft was re-vascularized on the abdomen.

Other groups reported, even very recently, few cases of abdominal wall transplantation[35] but the comprehensive picture of the results, related to the use of VCAs to close the abdominal wall after intestinal/multi-visceral transplantation ,were summarized in a recent paper published in 2017[24] where 35 full-thickness vascularized abdominal wall transplants were described (17 in Oxford, 12 in Miami, 3 in Bologna, 1 in Chennai, 1 in Indianapolis, 1 in Groningen).

The reported rate of successful abdominal closure after abdominal wall transplantation is very high, with 88% of flap/graft survival and no related mortality[26]: The overall follow-up is between 6 mo (Oxford, Bologna) and 7 years (Miami).

Moreover, it is worthwhile to notice that the skin component of the abdominal wall may serve as an immune modulator: A recent paper[37] analyzed a small cohort of 29 intestinal/multi-visceral transplants, 14 of them combined with abdominal wall transplants. The advantage to carry a wall graft was represented by lower bowel rejection rate (7% vs 27%) and lower rate (14% vs 33%) of misdiagnoses (viral infection vs rejection), followed by better intestinal graft survival (79% vs 60%).

Despite the good outcome, the procedure is still limited in few transplant centers where the expertise of the transplant team is well integrated with the plastic surgical service: Due to the low the numbers presented also by the 3 main groups (Miami, Oxford and Bologna) it is not possible to make a definitive statement related to the best technique (less morbidity, flap loss, and operative time).

Literature has shown that wall transplantation is feasible and reasonably time-consuming but it is a safe procedure with low morbidity and mortality.

CONCLUSION

The evolution and success of intestinal and multi-visceral transplantation has, in the last 20 years, raised the issue of difficult or even impossible abdominal closure, a topic very rarely encountered in other fields of transplantation.

The number of transplanted organs (volume) and/or graft edema, worsened by a small recipient abdominal cavity due to age or previous surgeries, makes a primary closure technically challenging or even impossible.

Different techniques have been proposed to address this topic and the choice depends upon the transplant team’s expertise and/or the availability of a plastic surgical service.

Whatever the approach used, may it be reduction of donor graft size or abdominal wall transplantation, it is important to realize that they may not be mutually exclusive to each other and both approaches can be used as a combination in the same recipient to assure the success of the transplant procedure.