Original Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Diabetes. May 15, 2012; 3(5): 94-104
Published online May 15, 2012. doi: 10.4239/wjd.v3.i5.94
Figure 5
Figure 5 Effects of curcumin on nuclear factor erythroid-2-related factor-2 signaling. A: The protein levels of nuclear factor erythroid-2-related factor-2 (Nrf2), heme oxygenase-1 (HO-1) in skeletal muscle (β-actin as the loading control of total protein); B: Nuclear Nrf2 contents in skeletal muscle were probed with Nrf2 antibody (Histone H3 as the nuclear protein loading control). C, D: The levels of Nrf2 and HO-1 in livers were examined by western blotting. The analysis of relative optical density on western blots was presented in the lower panels or the right panel for corresponding figures. The mice were fed with indicated diets and gavaged with curcumin or vehicle. At the end of 18 wk, the mice were sacrificed and tissues were collected for protein or nuclear preparation. Western blotting was performed with indicated antibodies. aP < 0.05, bP < 0.01, cP < 0.001, HFD or HFD+curcumin vs ND; dP < 0.05, HFD + curcumin vs HFD. HFD: High fat diet; ND: normal diet.