shRNA-interfering LSD1 inhibits proliferation and invasion of gastric cancer cells via VEGF-C/PI3K/AKT signaling pathway

Figure 1 Detection of Lysine Specific Demethylase 1 mRNA and protein levels in gastric cancer. A: Quantitative RT-PCR was used to detect expression of Lysine Specific Demethylase 1 (LSD1) mRNA in gastric cancer tissues; B: western blotting was used to detect expression level of LSD1 protein in gastric cancer tissues. ^aP < 0.05, ^bP < 0.01.

Figure 2 Survival analysis of relationship between Lysine Specific Demethylase 1 expression level and prognosis of gastric cancer patients. LSD1: Lysine Specific Demethylase 1.

Figure 3 Reverse transcription polymerase chain reaction and Western blotting detection of interference efficiency of Lysine Specific Demethylase 1-shRNA transfection. A: LSD1 mRNA expression was detected by reverse transcription polymerase chain reaction; B: protein expression was detected by Western blotting. LSD1: Lysine Specific Demethylase 1.

Figure 4 Effect of downregulating Lysine Specific Demethylase 1 expression on proliferation of MKN-45 cells. LSD1: Lysine Specific Demethylase 1.

Figure 5 Effect of downregulating Lysine Specific Demethylase 1 expression on the invasiveness of MKN-45 cells (×200). A: Control group; B: LSD1-shRNA transfection group. LSD1: Lysine Specific Demethylase 1.

Figure 6 Effect of downregulating Lysine Specific Demethylase 1 expression on expression of molecular proteins associated with MKN-45 cell transfer in VEGF-C/PI3K/AKT signaling pathw