Significance of MD-2 and MD-2B expression in rat liver during acute cholangitis

doi:10.4254/wjh.v2.i6.233

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Jun 27, 2010 (publication date) through Apr 25, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Jun 27, 2010; 2(6): 233-238
Published online Jun 27, 2010. doi: 10.4254/wjh.v2.i6.233

Table 1 Primers used for real-time PCR

	Forward	Reverse
MD-2 (130 bp)	5'-AAATCCCTATTTCAATTAGTTCTGAACC-3'	5'-GAGTTGATATTGATGAACAGGTTGAAAT-3'
MD-2B (93 bp)	5'-AAATCCCTATTTCAATTAGTTCTGAACC-3'	5'-GCTTCGGCAATTTTGGAATG-3'
TLR4 (117 bp)	5'-GATTGCTCAGACATGGCAGTTTC-3'	5'-CTGCTAAGAAGGCGATACAATTCG-3'
GAPDH (97 bp)	5'-CCGAGGGCCCACTAAAGG-3'	5'-GCTGTTGAAGTCACAGGAGACAA-3'

Table 2 TLR4 expression in rat liver tissue (mean ± SE) (copy ratio 10⁴)

	6 h	12 h	24 h	48 h	72 h
Group A	4.32 ± 0.721	4.17 ± 0.708	4.40 ± 0.435	4.36 ± 0.396	4.48 ± 0.504
Group B	3.96 ± 0.051	4.90 ± 0.075^a	8.52 ± 0.023^a	11.11 ± 0.029^a	12.90 ± 0.042^a
Group C	3.87 ± 0.031	5.23 ± 0.033^a^c	9.87 ± 0.025^a^c	13.70 ± 0.115^a^c	19.70 ± 0.123^a^c
Group D	0.16 ± 0.003^e	0.17 ± 0.002^e	0.22 ± 0.002^e	0.58 ± 0.001^e	0.77 ± 0.006^e

^aP < 0.05 vs the same group at different time points;

^cP < 0.05 vs group B at the same time;

^eP < 0.05 vs group C at the same time.

Table 3 MD-2 expression in rat liver tissue (mean ± SE) (copy ratio 10³)

	6h	12h	24h	48h	72h
Group A	1.16 ± 0.042	1.17 ± 0.057	1.14 ± 0.016	1.16 ± 0.028	1.17 ± 0.048
Group B	1.15 ± 0.015	2.11 ± 0.042^a	3.29 ± 0.034^a	15.41 ± 0.267^a	21.97 ± 0.271^a
Group C	1.17 ± 0.028	4.10 ± 0.054^a^c	12.27 ± 0.078^a^c	21.158 ± 0.185^a^c	30.21 ± 0.342^a^c
Group D	0.33 ± 0.017^e	0.40 ± 0.061^e	1.15 ± 0.025^e	3.61 ± 0.089^e	4.58 ± 0.034^e

^aP < 0.05 vs the same group at different time points;

^cP < 0.05 vs group B at the same time;

^eP < 0.05 vs group C at the same time.

Table 4 MD-2B expression in rat liver tissue (mean ± SE) (copy ratio 10²)

	6h	12h	24h	48h	72h
Group A	2.35 ± 0.013	2.38 ± 0.013	2.35 ± 0.012	2.35 ± 0.012	2.35 ± 0.010
Group B	2.47 ± 0.021	1.32 ± 0.035^a	0.98 ± 0.006^a	0.71 ± 0.006^a	0.48 ± 0.002^a
Group C	2.47 ± 0.021	1.42 ± 0.015^a^c	0.26 ± 0.002^a^c	0.09 ± 0.001^a^c	0.07 ± 0.003^a^c
Group D	6.03 ± 0.015^e	4.50 ± 0.017^e	3.85 ± 0.015^e	3.62 ± 0.015^e	2.39 ± 0.022^e

^aP < 0.05 vs the same group at different time points;

^cP < 0.05 vs group B at the same time;

^eP < 0.05 vs group C at the same time.

Citation: Miao HL, Qiu ZD, Hao FL, Bi YH, Li MY, Chen M, Chen NP, Zhou F. Significance of MD-2 and MD-2B expression in rat liver during acute cholangitis. World J Hepatol 2010; 2(6): 233-238
URL: https://www.wjgnet.com/1948-5182/full/v2/i6/233.htm
DOI: https://dx.doi.org/10.4254/wjh.v2.i6.233