Promotive action of 2-acetylaminofluorene on hepatic precancerous lesions initiated by diethylnitrosamine in rats: Molecular study

doi:10.4254/wjh.v13.i3.328

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Mar 27, 2021 (publication date) through Apr 25, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Mar 27, 2021; 13(3): 328-342
Published online Mar 27, 2021. doi: 10.4254/wjh.v13.i3.328

Table 1 Expression rate of hepatocytes positive for proliferating cell nuclear antigen was calculated as number of positive field expression in 10 fields per rat liver tissue

Group	10 wk duration	16 wk duration
DEN	+	++
DEN + 100 AAF	+	++
DEN + 200 AAF	++	+++
DEN + 300 AAF	++	+++

+: Positive expression found in 1-3 fields; ++: Positive expression found in 4-6 fields; +++: Positive expression found in 7-10 field. DEN: Diethylnitrosamine; AAF: Acetylaminofluorene.

Table 2 Effect of diethylnitrosamine and 2-acetylaminofluorene on alpha-fetoprotein and liver function

	AFP	ALT	Total bilirubin	Direct bilirubin	Albumin
Naïve	22.8 ± 1.13	33.3 ± 6.83	0.30 ± 0.18	0.27 ± 0.14	3.77 ± 0.23
Week 10
DEN	89.2 ± 28.8^d	63.0 ± 27.5^d	1.44 ± 0.45^d	1.03 ± 0.14^d	2.49 ± 0.15^d
DEN + 100 AAF	116 ± 52.1^d	78.3 ± 17.8^d	2.07 ± 0.44^d^,^e	1.40 ± 0.39^d	2.83 ± 0.19^d
DEN + 200 AAF	223 ± 124^b^,^d	82.7 ± 12.7^d	2.73 ± 0.23^a^,^d^,^e	1.67 ± 0.19^a^,^d^,^e	2.73 ± 0.14^d
DEN + 300 AAF	305 ± 126^d^,^e	98.0 ± 10.7^d^,^e	3.13 ± 0.36^b^,^d^,^e	2.13 ± 0.61^d^,^e	3.15 ± 0.38^c^,^d^,^e
Week 16
DEN	159 ± 32.2^d	94.1 ± 6.4^d	2.13 ± 0.55^d	1.60 ± 0.39^d	2.0 ± 0.62^d
DEN + 100 AAF	290 ± 241^d	104 ± 31.9^d	2.23 ± 0.36^d	2.25 ± 0.63^d	2.57 ± 0.37^d
DEN + 200 AAF	815 ± 143^a^,^d^,^f	128 ± 36.9^d	4.10 ± 0.39^a^,^d^,^f	2.53 ± 0.63^a^,^d^,^f	2.17 ± 0.29^d
DEN + 300 AAF	1059 ± 360^b^,^d^,^f	210 ± 63.2^b^,^c^,^d^,^f	4.47 ± 0.99^b^,^d^,^f	3.10 ± 0.39^d^,^f	2.13 ± 0.67^b^,^c^,^d^,^f

Values are mean ± SD; number of animals = 6 rats/each group.

^aP < 0.05 when DEN + 200 acetylaminofluorene (AAF) is compared to the DEN + 100 AAF.

^bP < 0.05 when DEN + 300 AAF is compared to the DEN + 100 AAF.

^cP < 0.05 when DEN + 300 AAF is compared to the DEN + 200 AAF.

^dP < 0.05 compared to the naïve group.

^eP < 0.05 compared to the diethylnitrosamine (DEN) group at week 10 group.

^fP < 0.05 compared to the DEN at week 16 group. One-way ANOVA followed by Tukey's multiple comparison test. DEN: Diethylnitrosamine; 2-AAF: 2-Acetylaminofluorene; AFP: Alpha-fetoprotein; ALT: Alanine aminotransferase.

Table 3 Effect of diethylnitrosamine and 2-acetylaminofluorene on relative quantification of lncRNA-RP11-513I15.6 (long-noncoding RNA), relative quantification of miR-1262 and relative quantification of miR-1298 (microRNA)

	RQ of lncRNA-RP11-513I15.6		RQ of miR-1262		RQ of miR-1298
	Liver	Serum	Liver	Serum	Liver	Serum
Naïve	2.33 ± 0.31	1.86 ± 0.41	0.38 ± 0.09	0.26 ± 0.34	0.77 ± 0.26	0.1 ± 0.04
Week 10
DEN	1.31 ± 0.36^d	0.99 ± 0.15^d	1.63 ± 0.28^d	1.40 ± 0.13^d	1.55 ± 0.37^d	1.08 ± 0.11^d
DEN + 100 AAF	1.03 ± 0.2^d	0.63 ± 0.13^d^,^e	2.26 ± 0.54^d	2.23 ± 0.19^d	1.85 ± 0.12^d	1.48 ± 0.56^d
DEN + 200 AAF	0.58 ± 0.12^a^,^d^,^e	0.44 ± 0.05^d^,^e	2.52 ± 0.44^d^,^e	2.81 ± 0.49^d^,^e	2.46 ± 0.37^d^,^e	1.82 ± 0.47^b^,^c^,^d^,^e
DEN + 300 AAF	0.47 ± 0.037^b^,^d^,^e	0.39 ± 0.005^d^,^e	3.9 ± 0.36^d^,^e	3.59 ± 1.10^b^,^d^,^e	2.88 ± 0.11^d^,^e	3.30 ± 0.18^d^,^e
Week 16
DEN	0.76 ± 0.1^d	0.52 ± 0.13^d	3.12 ± 0.62^d	2.15 ± 0.08^d	2.52 ± 0.56^d	2.23 ± 0.26^d
DEN + 100 AAF	0.46 ± 0.04^d^,^f	0.22 ± 0.04^d	4.48 ± 0.63^d^,^f	4.08 ± 0.32^d	3.92 ± 0.61^d^,^f	4.56 ± 0.61^d^,^f
DEN + 200 AAF	0.26 ± 0.07^d^,^f	0.14 ± 0.07^d^,^f	5.71 ± 0.76^a^,^d^,^f	7.38 ± 2.24^d^,^f	4.58 ± 0.56^d^,^f	5.78 ± 1.72^d^,^f
DEN + 300 AAF	0.14 ± 0.04^b^,^d^,^f	0.12 ± 0.06^d^,^f	6.45 ± 1.04^b^,^d^,^f	9.78 ± 4.32^b^,^d^,^f	5.89 ± 1.27^d^,^f	7.38 ± 2.05^b^,^d^,^f