Preserved liver regeneration capacity after partial hepatectomy in rats with non-alcoholic steatohepatitis

Table 1 Biochemistry and measurements

	Standard diet			High fat high cholesterol diet
	NH (n = 9)	PH-2 (n = 9)	PH-5 (n = 8)	NH (n = 9)	PH-2 (n = 9)	PH-5 (n = 8)
Weight at euthanisation, g	294 ± 22	289 ± 18	305 ± 12	289 ± 21	271 ± 181	290 ± 15
Liver weight at euthanisation, g	8.1 ± 0.6	6.1 ± 0.5	7.5 ± 1.3	15.4 ± 2.01	9.0 ± 0.81	12.3 ± 1.71
Recected liver at PH, g	-	5.2 ± 0.3	5.0 ± 0.5	-	11.2 ± 2.01	11.4 ± 0.71
Hepatic regeneration ratio, %	1.0	0.82 ± 0.08	1.04 ± 0.17	1.0	0.61 ± 0.11	0.77 ± 0.121
Net regeneration (g)	-	3.8 ± 0.5	4.2 ± 1.2	-	5.3 ± 0.9	7.4 ± 1.61
Total protein, g	0.83 ± 0.11	0.51 ± 0.08	0.69 ± 0.18	1.10 ± 0.13	0.60 ± 0.12	0.90 ± 0.14
Ki-67 Index, positive profiles, mm2	1.6 ± 0.6	216 ± 83.2	27.2 ± 22.3	9.0 ± 3.21	227.5 ± 80	31.7 ± 11.1
Spleen weigth at euthanisation, g	0.64 ± 0.12	0.83 ± 0.12	1.10 ± 0.16	1.25 ± 0.361	1.04 ± 0.31	1.34 ± 0.44
Alanine transaminase, u/L	24.1 ± 5.2	64.9 ± 9.0	28.8 ± 13.4	79.9 ± 27.41	130.1 ± 25.41	45.8 ± 20.5
ΔAlanine transaminase, u/L	-	41 ± 9	5 ± 13	-	50 ± 25	-34 ± 211
Albumin (g/L)	19.0 ± 1.9	14.7 ± 1.0	12.4 ± 1.5	15.1 ± 1.91	12.2 ± 0.8	11.5 ± 2.3
α2-macroglobulin, μg/mL	18.1 ± 0.7	102 ± 12.6	98.2 ± 13	22.6 ± 3.41	157.8 ± 401	94.5 ± 64.5
Prothrombin-proconvertin ratio	0.32 ± 0.03	0.33 ± 0.04	0.32 ± 0.03	0.34 ± 0.03	0.38 ± 0.06	0.33 ± 0.03

Mean ± SD,

¹P < 0.05. NH: No-hepatectomy; PH-2: Partial hepatectomy and 2 d; PH-5: Partial hepatectomy and 5 d.

Table 2 Average scores of the Kleiner criteria and the final histological grading of both the Kleiner and Bedossa criteria

	High fat high cholesterol diet
	NH (n = 9)	PH-2 (n = 9)	PH-5 (n = 8)
Kleiner criteria score
Steatosis	3	3	3
Ballooning	0.6	0.7	0.5
Inflammation	2.7	1.6	0.8
Fibrosis	0	0	0
NAS score	6.2	5.2	4.3
Histological grading
Borderline NASH/NASH, Kleiner criteria	1/8	4/5	4/4
NAFLD/NASH, Bedossa criteria	3/6	4/5	4/4

NASH: Non-alcoholic steatohepatitis; NAFLD: Non-alcoholic fatty liver disease; NH: No-hepatectomy; PH-2: Partial hepatectomy and 2 d; PH-5: Partial hepatectomy and 5 d.