Preserved liver regeneration capacity after partial hepatectomy in rats with non-alcoholic steatohepatitis

Figure 1 Overview of the groups, 9 animals in each group. PH-2: Partial hepatectomy and 2 d; PH-5: Partial hepatectomy and 5 d.

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Figure 2 Examples of liver histology. A and C shows Hematoxylin and Eosin; B and D shows Masson Trichome; A and B are from an animal fed the standard diet and euthanised without hepatectomy; C and D are from an animal fed the high fat high cholesterol diet and euthanised without hepatectomy.

Figure 3 Examples of liver histology. A and C shows Hematoxylin and Eosin; B and D shows Masson Trichome; A and B are from an animal fed the standard diet and euthanised two days after hepatectomy; C and D are from an animal fed the high fat high cholesterol diet and euthanised two days after hepatectomy.

Figure 4 Examples of liver histology. A and C shows Hematoxylin and Eosin; B and D shows Masson Trichome; A and B are from an animal fed the standard diet and euthanised five days after hepatectomy; C and D are from an animal fed the high fat high cholesterol diet and euthanised five days after hepatectomy.

Figure 5 Total number of Ki-67 positive hepatocytes (A); regenerated protein ratio (B); Alanine transaminase (C) and Bilirubin (D). Means and standard derivation displayed. ^aP < 0.05 compared to respective standard diet group. NH: No hepatectomy; PH-2: Partiel hepatectomy and 2 d; PH-5: Partiel hepatectomy and 5 d.

Figure 6 Relative mRNA expression. A: Tumor necrosis factor α (TNFα); B: Interleukin-6 (IL-6) standardized to glyceraldehyde 3-phosphate dehydrogenase. Means and standard derivation displayed. Statistical analysis made using the ANOVA test, ^aP < 0.05 compared to respective standard diet group. NH: No hepatectomy; PH-2: Partiel hepatectomy and 2 d; PH-5: Partiel hepatectomy and 5 d.

Figure 7 Relative mRNA expression. A: Transforming Growth Factor β (TGFβ); B: Connective Tissue Growth Factor (CTGF); and C: Collagen 1α1 standardized (Col1α1) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Means and standard derivation displayed. Statistical analysis made using the ANOVA test, ^aP < 0.05 compared to respective standard diet group. NH: No hepatectomy; PH-2: Partiel hepatectomy and 2 d; PH-5: Partiel hepatectomy and 5 d.

Figure 8 Relative mRNA expression. A: Hepatocyte growth factor (HGF); B: Proto-oncogene, tyrosine kinase (MET); C: Epidermal growth factor (EGF); and D: Transforming growth factor α (TGFα), standardized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Means and standard derivation displayed. Statistical analysis made using the ANOVA test, ^aP < 0.05 compared to respective standard diet group. NH: No hepatectomy; PH-2: Partiel hepatectomy and 2 d; PH-5: Partiel hepatectomy and 5 d.