Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice
Kandy T Velázquez, Reilly T Enos, Jackie E Bader, Alexander T Sougiannis, Meredith S Carson, Ioulia Chatzistamou, James A Carson, Prakash S Nagarkatti, Mitzi Nagarkatti, E Angela Murphy, Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, Columbia, SC 29209, United States
James A Carson, College of Health Professions, University of Tennessee Health Sciences Center, Memphis, TN 38163, United States
Author contributions: Velázquez KT performed the majority of experiments and analyzed the data; Chatzistamou I performed histopathological analysis; Velázquez KT performed staining; Velázquez KT, Enos RT, Carson MS, and Sougiannis AT performed molecular investigations; Velázquez KT, Bader JE, Nagarkatti PS, Nagarkatti M and Murphy EA designed and coordinated the microbiome research; Velázquez KT, Enos RT, Carson JA and Murphy EA designed and coordinated the obesity and non-alcoholic fatty liver disease research; Velázquez KT, Enos RT, and Murphy EA wrote the paper; all authors were involved in editing the manuscript.
Supported by National Institute of Health, No. NCI-3R01CA121249-08S1, NCCIH-K99AT009206 and No. NCI-1R21CA191966.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee at the University of South Carolina.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: Data from this manuscript will be available upon request.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: E Angela Murphy, PhD, Associate Professor, Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, 6439 Garners Ferry Rd, Columbia, SC 29209, United States. firstname.lastname@example.org
Telephone: +1-803-2163414 Fax: +1-803-2163414
Received: March 7, 2019
Peer-review started: March 11, 2019
First decision: April 11, 2019
Revised: July 5, 2019
Accepted: July 16, 2019
Article in press: July 17, 2019
Published online: August 27, 2019