Published online Sep 8, 2016. doi: 10.4254/wjh.v8.i25.1067
Peer-review started: March 31, 2016
First decision: June 12, 2016
Revised: June 28, 2016
Accepted: August 11, 2016
Article in press: August 15, 2016
Published online: September 8, 2016
To analyze the association of the CD36 polymorphism (rs1761667) with dietary intake and liver fibrosis (LF) in chronic hepatitis C (CHC) patients.
In this study, 73 patients with CHC were recruited. The CD36 genotype (G > A) was determined by a TaqMan real-time PCR system. Dietary assessment was carried out using a three-day food record to register the daily intake of macronutrients. Serum lipids and liver enzymes were measured by a dry chemistry assay. LF evaluated by transient elastography (Fibroscan®) and APRI score was classified as mild LF (F1-F2) and advanced LF (F3-F4).
Overall, the CD36 genotypic frequencies were AA (30.1%), AG (54.8%), and GG (15.1%), whereas the allelic A and G frequencies were 57.5% and 42.5%, respectively. CHC patients who were carriers of the CD36 AA genotype had a higher intake of calories attributable to total fat and saturated fatty acids than those with the non-AA genotypes. Additionally, aspartate aminotransferase (AST) serum values were higher in AA genotype carriers compared to non-AA carriers (91.7 IU/L vs 69.8 IU/L, P = 0.02). Moreover, the AA genotype was associated with an increase of 30.23 IU/L of AST (β = 30.23, 95%CI: 9.0-51.46, P = 0.006). Likewise, the AA genotype was associated with advanced LF compared to the AG (OR = 3.60, 95%CI: 1.16-11.15, P = 0.02) or AG + GG genotypes (OR = 3.52, 95%CI: 1.18-10.45, P = 0.02).
This study suggests that the CD36 (rs1761667) AA genotype is associated with higher fat intake and more instances of advanced LF in CHC patients.
Core tip: In this study, chronically infected hepatitis C patients who were carriers of the AA genotype of the CD36 receptor polymorphism (rs1761667) showed a higher risk of advanced liver fibrosis compared to patients with an AG/GG genotype. This liver damage was associated with the consumption of a hepatopatogenic diet, high-calories and excessive intake of total and saturated fat, typical of the population of West Mexico. Thus, preventive nutritional intervention strategies based on the CD36 genotype may be a useful tool to avoid further liver damage due to alterations in liver lipid metabolism and inflammation in patients with chronic hepatitis C infection.