Brief Article
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World J Hepatol. Nov 27, 2013; 5(11): 642-648
Published online Nov 27, 2013. doi: 10.4254/wjh.v5.i11.642
Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors in living donor liver transplantation
King-Wah Chiu, Toshiaki Nakano, Kuang-Den Chen, Li-Wen Hsu, Chia-Yun Lai, Ho-Ching Chiu, Ching-Yin Huang, Yu-Fan Cheng, Shigeru Goto, Chao-Long Chen
King-Wah Chiu, Toshiaki Nakano, Kuang-Den Chen, Li-Wen Hsu, Chia-Yun Lai, Ho-Ching Chiu, Ching-Yin Huang, Yu-Fan Cheng, Shigeru Goto, Chao-Long Chen, Liver Transplant Program, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 833, Taiwan
Author contributions: Chiu KW and Chen CL designed research; Nakano T and Chen KD performed research; Hsu LW, Lai CY, Chiu HC and Huang CY contributed new reagents or analytic tools; Cheng YF and Goto S analyzed data; Chiu KW and Nakano T wrote the paper.
Supported by A grant from Chang Gung Memorial Hospital, CMRPG8A0631 to Chiu KW of Taiwan
Correspondence to: Chao-Long Chen, MD, Liver Transplant Program, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, 123 Ta-Pei Road, Niao-Sung, Kaohsiung 833, Taiwan. chchen@adm.cgmh.org.tw
Telephone: +886-7-7317123 Fax: +886-7-7336856
Received: August 11, 2013
Revised: September 28, 2013
Accepted: October 17, 2013
Published online: November 27, 2013
Abstract

AIM: To investigate the evidence of homogeneous phenomenon on CYP3A5*3 MDR1-3435 and CYP3A4*18 of the liver graft after living donor liver transplantation (LDLT).

METHODS: We identified the proportional change of the CYP3A5*3, MDR1-3435 and CYP3A4*18 from the peripheral blood mononuclear cell of 41 pairs recipient/donor with different genotype polymorphisms and 119 liver graft biopsy samples used with the pyrosequencing technique after LDLT. Polymerase chain reaction/ligase detection reaction assay and restriction fragment length polymorphism was employed for genotyping the CYP3A5*3 and CYP3A4*18 single nucleotide polymorphisms (SNPs). All of the recipients and donors expressed with the similar SNP genotype of CYP3A5*3, MDR1-3435 or CYP3A4*18 were excluded.

RESULTS: The final genetic polymorphisms of the liver graft biopsy samples of CYP3A5*3, MDR1-3435 and CYP3A4*18 was predominated depends on the donor with restriction fragment length polymorphism and seems to be less related to the recipient. The proportional changes of G to A alleles of the 119 samples of CYP3A5*3 (included A > A/G, A/G > A, A/G > G, G > A, G > A/G and A > G), C to T alleles of the 108 samples of MDR1-3435 (included C > C/T, C/T > C, C/T > T, T > C/T and T > C), and T to C alleles of 15 samples of CYP3A4*18 (included T/C > T and T > C/T) were significant different between the recipients and the liver graft biopsy samples (P < 0.0001) and less difference when compared with the donors in the pyrosequencing analysis after LDLT.

CONCLUSION: The CYP3A5*3, MDR1-3435 and CYP3A4*18 of the recipient could be modified by the donor so-called homogenous phenomenon when the recipient’s blood drained into the liver graft.

Keywords: Pyrosequencing, CYP3A5*3, MDR1-3435, CYP3A4*18, Liver biopsy, Living donor liver transplantation

Core tip: The most innovative concept is that pyrosequencing can deeply clarify the proportional change of the A and G alleles in CYP3A5*3, C and T alleles in MDR1-3435, and T and C alleles in CYP3A4*1 when the different genotype of single nucleotide polymorphism after living donor liver transplantation. The biogenetic characteristic of the recipient could be modified by a donor you want to change the genetic characteristic. For further confirmation, homogeneous phenomenon was the truly occurred in the cytochrome P450 system when the recipients and donors with different genotype of the single nucleotide polymorphism.