Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity

doi:10.4252/wjsc.v9.i9.159

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Sep 26, 2017; 9(9): 159-168
Published online Sep 26, 2017. doi: 10.4252/wjsc.v9.i9.159

Table 1 List of primers for quantitative reverse transcriptase-PCR

Primer	Sequence (5’-3’)
ActB forward	ATGGAGGGGAATACAGCCC
ActB reverse	TTCTTTGCAGCTCCTTCGTT
Klf4 forward	CAGTGGTAAGGTTTCTCGCC
Klf4 reverse	GCCACCCACACTTGTGACTA
Bmi1 forward	TGGTTGTTCGATGCATTTCT
Bmi1 reverse	CTTTCATTGTCTTTTCCGCC
Cd44 forward	AGCGGCAGGTTACATTCAAA
Cd44 reverse	CAAGTTTTGGTGGCACACAG
E-Cadherin forward	AAAAGAAGGCTGTCCTTGGC
E-Cadherin reverse	GAGGTCTACACCTTCCCGGT
ZO-1 forward	CCTGTGAAGCGTCACTGTGT
ZO-1 reverse	CGCGGAGAGAGACAAGATGT
Vimentin forward	AGAGAGAGGAAGCCGAAAGC
Vimentin reverse	TCCACTTTCCGTTCAAGGTC
Fibronectin forward	ACTGGATGGGGTGGGAAT
Fibronectin reverse	GGAGTGGCACTGTCAACCTC
Snai1 forward	AGTGGGAGCAGGAGAATGG
Snai1 reverse	CTTGTGTCTGCACGACCTGT
Snai2 forward	GATGTGCCCTCAGGTTTGAT
Snai2 reverse	GGCTGCTTCAAGGACACATT
Zeb1 forward	TCATCGGAATCTGAATTTGC
Zeb1 reverse	CCAGGTGTAAGCGCAGAAAG
Zeb2 forward	TGCGTCCACTACGTTGTCAT
Zeb2 reverse	TCTTATCAATGAAGCAGCCG
Twist1 forward	CATGTCCGCGTCCCACTA
Twist1 reverse	TCCATTTTCTCCTTCTCTGGA
Twist2 forward	GCCTGAGATGTGCAGGTG
Twist2 reverse	GTCTCAGCTACGCCTTCTCC

Citation: Jayachandran A, Shrestha R, Dhungel B, Huang IT, Vasconcelos MYK, Morrison BJ, Ramlogan-Steel CA, Steel JC. Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity. World J Stem Cells 2017; 9(9): 159-168
URL: https://www.wjgnet.com/1948-0210/full/v9/i9/159.htm
DOI: https://dx.doi.org/10.4252/wjsc.v9.i9.159