Mesenchymal stem cells and collagen patches for anterior cruciate ligament repair

Table 1 Real-time polymerase chain reaction primers used for real-time polymerase chain reaction

Abbreviation	Gene name	Forward	Reverse
18S	18S Ribosomal RNA	CGA TGC GGC GGC GTT ATT C	TCT GTC AAT CCT GTC CGT GTC C
HsACAN	Core protein	CAT CAC TGC AGC TGT CAC	AGC AGC ACT ACC TCC TTC
Hs_col1	Collagen 1 A2	GTG GCA GTG ATG GAA GTG	CAC CAG TAA GGC CGT TTG
Hs_col2	Collagen 2 A1	AGCAAGAGCAAGGAGAAG	GGGAGCCAGATTGTCATC
Hs_col3	Collagen 3 A1	GTG AGC CTG GTA AGA ATG G	CCT GGA ACA CCT GGA ATA C
Hs_TNC	Tenascin C	CAC GCT GAG GTT GAT GTT C	GTT GAT GGT CGC TGG ATT G
Hs_SCX	Scleraxis A	CAC CAA CAG CGT GAA CAC	GCA GCG TCT CAA TCT TGG A
Hs_TNMD	Tenomodulin	ACA AGC AAG TGA GGA AGA A	GAC GGC AGT AAA TAC AAC AAT
Hs_MMP13	Collagenase 3	AGT GGT GGT GAT GAA GAT	CTA AGG TGT TAT CGT CAA GTT
Hs_MMP3	Matrix metallopeptidase 3	CAA GGC ATA GAG ACA ACA TAG A	GCA CAG CAA CAG TAG GAT

All primers were run at 61 °C Ta (annealing temperature) and a two-step protocol (95 °C for 10 s and 61 °C for 30 s) and 45 cycles.

Table 2 Summary of the donor tissue that was used to harvest and expand primary cells, i.e., bone marrow derived mesenchymal stem cells and anterior cruciate ligament-derived tenocytes and stage of passage for 3D culture experiment

MSCs donor characteristics						TCs donor characteristics
Experiment	ID	Passage	Donor gender	Donor age	Source	Experiment	ID	Passage	Donor gender	Donor age	Source
1	62	1	m	75	Lumbar	1	66	1	f	62	ACL
2	62	2	m	75	Lumbar	2	66	2	f	62	ACL
3	65	1	f	84	Lower limbs	3	67	1	f	38	ACL
4	47	2	m	64	Thoracal	4	67	2	f	38	ACL
5	48	2	m	55	Thoracal	5	70	1	f	74	ACL

MSC: Mesenchymal stem cell; TC: Tenocyte; ACL: Anterior cruciate ligament.