Copyright ©The Author(s) 2020.
World J Stem Cells. Aug 26, 2020; 12(8): 761-775
Published online Aug 26, 2020. doi: 10.4252/wjsc.v12.i8.761
Table 1 The use of pluripotent stem cells as in vitro models for studying the developmental origins of health and diseases
Environmental insult(s)Type of pluripotent stem cellsCell lineageEffects on differentiationRef.
Hyperglycemia (25-50 mmol/L)hESCPancreatic(1) Inhibited differentiation into DE; and (2) retained repressive H3K27me3 mark on DE marker promoters[85]
Hyperglycemia (25-50 mmol/L)mESCPancreaticInhibited differentiation into DE[85]
TCDD (10 pmol/L)hESCPancreaticDysregulated DNA methylome of genes related to diabetes[99]
TCDD (10-100 pmol/L)hESCPancreatic(1) Dysregulated DNA methylome of genes related to insulin signaling and diabetes; (2) inhibited differentiation into pancreatic progenitor; and (3) promoted DNA hypermethylation of PRKAG1[100]
Hyperglycemia (10 mmol/L), endothelin-1 (ET-1) (10 nmol/L), and cortisol (1 μmol/L)hiPSCCardiac(1) Inhibited cardiomyocyte differentiation; and (2) elevated oxidative stress in cardiomyocytes formed[114]
Hyperglycemia (25 mmol/L)mESCCardiacInhibited mesoderm and subsequent cardiomyocyte differentiation[117]
Hyperglycemia (25 mmol/L)mESCCardiacEnhanced cardiomyocyte differentiation[118]
TCDD (1 nmol/L)mESCCardiacInhibited cardiomyocyte differentiation[136]
TCDD (10-100 pmol/L)hESCCardiacDysregulated DNA methylome of genes related to cardiomyopathy[100]
BPA (1-8 μg/mL); PFOS (5-40 μg/mL); PFOA (10-80 μg/mL)mESCCardiacInhibited cardiomyocyte differentiation[137]
Hyperglycemia (25 mmol/L)mESCNeuralInhibited neural differentiation[125]