Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells

Figure 5 Cell growth and gene expression kinetics of HES-3 cells grown in cultures with and without supplements. Cell densities at multiple times points over a period of 10 to 16 d were recorded with cultures with and without supplements bovine serum albumin (BSA) (A); Soy hydrolysate (Hysoy) (B). Gene expression profile recorded via quantitative real-time reverse transcription-polymerase chain reaction at multiple time points over 16 d during the course of cardiomyocytes (CM) differentiation. 6 genes: hESC pluripotency (OCT4, Nanog), Mesoderm (Mesp1, T-bra), cardiac progenitors (Mkx2.5), and cardiomyocytes (MHC) were monitored for BSA (C); Hysoy (D). n = 3, ^aP < 0.05, ^bP < 0.01 vs SB medium.