Original Article
Copyright ©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Stem Cells. Jul 26, 2013; 5(3): 86-97
Published online Jul 26, 2013. doi: 10.4252/wjsc.v5.i3.86
Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells
Sherwin Ting, Marti Lecina, Yau-Chi Chan, Hung Fat Tse, Shaul Reuveny, Steve KW Oh
Sherwin Ting, Marti Lecina, Shaul Reuveny, Steve KW Oh, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), Singapore 138668, Singapore
Yau-Chi Chan, Hung Fat Tse, Cardiology Division, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China
Author contributions: Ting S and Lecina M contributed to conception and design; Ting S, Lecina M, and Chan YC contributed to collection and assembly of data; Ting S, Reuveny S and Reuveny S contributed to manuscript writing; all authors contributed to data analysis and interpretation, and final approval of manuscript.
Supported by Science Technology and Research (A*STAR); Hong Hong Kong Research Grant Council Collaborative Research Fund (HKU8/CRF/09) and Theme-based Research Scheme (T12-705/11); Tse HT and Oh SKW contributed to financial support
Correspondence to: Dr. Steve KW Oh, Principal Scientist, Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way No. 06-01, Singapore 138668, Singapore. steve_oh@bti.a-star.edu.sg
Telephone: +65-6407-0855 Fax: +65-6478-9561
Received: March 26, 2013
Revised: June 3, 2013
Accepted: June 8, 2013
Published online: July 26, 2013
Core Tip

Core tip: Nutrient supplements were screened for improving cell survival during the cardiomyocyte differentiation process of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a serum-free medium based on the inhibition of p38 MAPK (SB media). Soy hydrolysate and bovine serum albumin supplementation was found to improve cell viability and therefore increased the yield of HES-3 cardiomyocytes by 2.6-fold over non-supplemented SB medium. The enhancing effect of this medium was demonstrated in an additional hESC line (H1) and Siu1-hiPSC cell line (15 and 9 fold respectively). The cardiomyocytes formed expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like.