Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca, ICR and Balb/c mice

Figure 1 Morphology of mouse bone marrow cultures. Cells shown here represent passages 0-19 of bone marrow cells from CBA/Ca, ICR and Balb/c mice. Original magnification: × 200.

Figure 2 Bone marrow cultures acquire CD106⁺CD45^- immunophenotype at later passages. Bottom right quadrants show percentage of CD106⁺CD45^- cells for mesenchymal stem cells of CBA/Ca, ICR and Balb/c mice. Numbers in the upper right region indicate passage number.

Figure 3 Bone marrow cultures show decreased CD11b and increased Sca-1 expression at later passages. Histograms show the shift of CD11b⁺ at earlier passages to CD11b^- at later passages for each strain of mice. Expression of Sca-1 is increased with mesenchymal stem cells passages.

Figure 4 Mesenchymal stem cells cultures from all 3 strains of mice show positivity to CD44 and MHC I but negativity to MHC II. Numbers within plots indicate the percentage of positivity to each marker.

Figure 5 Mesenchymal stem cells from all 3 strains of mice differentiate into adipocytes and osteocytes. First and second row: images of non-induced mesenchymal stem cells (MSC) control and MSC differentiated into adipocytes, stained with Oil Red O. Third and fourth row: images of non-induced MSC control and MSC differentiated into osteocytes, stained with Alizarin Red S. Differences in quality of images are because they were taken using different camera/microscope systems.