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World J Stem Cells. Nov 26, 2014; 6(5): 651-657
Published online Nov 26, 2014. doi: 10.4252/wjsc.v6.i5.651
Published online Nov 26, 2014. doi: 10.4252/wjsc.v6.i5.651
Pluripotent stem cell-derived neural stem cells: From basic research to applications
Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan
Takashi Nakayama, Department of Biochemistry, Yokohama City University School of Medicine, Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
Nobuo Inoue, Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan
Author contributions: Otsu M and Inoue N drafted the article; Otsu M wrote this manuscript; Otsu M, Nakayama T and Inoue N revised this manuscript critically for important intellectual content.
Supported by Grant-in-Aid for Young Scientists (B), No. 24791230; Research Grant for long-range research initiative from JCIA; Selective Research Fund of Tokyo Metropolitan University and a Grant-in-Aid for Scientific Research, No. 20500339
Correspondence to: Nobuo Inoue, PhD, Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan. jun-inoue@bg8.so-net.ne.jp
Telephone: +81-3-38197382 Fax: +81-3-38191406
Received: July 23, 2014
Revised: September 4, 2014
Accepted: September 16, 2014
Published online: November 26, 2014
Revised: September 4, 2014
Accepted: September 16, 2014
Published online: November 26, 2014
Core Tip
Core tip:In vitro techniques for manipulating stem cells can enhance the development of stem cell-based therapies and effective prevention against human diseases. This review summarizes the techniques required to generate neural cells from pluripotent stem cells, as well as focusing on current research applications of a simple neuronal differentiation method, the neural stem sphere method.