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World J Stem Cells. Apr 26, 2014; 6(2): 248-255
Published online Apr 26, 2014. doi: 10.4252/wjsc.v6.i2.248
Published online Apr 26, 2014. doi: 10.4252/wjsc.v6.i2.248
Embryonic stem cell-derived neural progenitors as non-tumorigenic source for dopaminergic neurons
Mei-Chih Liao, Mihaela Diaconu, Ahmed Mansouri, Department of Molecular Cell Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany
Sebastian Monecke, Ralf Dressel, Department of Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany
Patrick Collombat, Univerité Nice Sophia Antipolis, UMR INSERM 1091/CNRS 7277/UNS, Diabetes Genetics Team, Institut de Biologie Valrose, 06100 Nice, France
Charles Timaeus, Walter Paulus, Ahmed Mansouri, Department of Clinical Neurophysiology, University Medical Center Göttingen, 37075 Göttingen, Germany
Tanja Kuhlmann, Institute of Neuropathology, University of Münster, 48149 Münster, Germany
Claudia Trenkwalder, Paracelsus-Elena-Klinik, 34128 Kassel, Germany
Patrick Collombat, Ahmed Mansouri, Genome and Stem Cell Center, GENKOK, Erciyes University, 38039 Kayseri, Turkey
Author contributions: Liao MC, Diaconu M, Monecke S, Collombat P, Timaeus C, Kuhlmann T, Dressel R and Mansouri A performed the experiments; Paulus W and Trenkwalder C contributed to the conception of the study; Dressel R and Mansouri A designed the study, wrote the manuscript and share senior authorship; all the authors approved the final version of the manuscript.
Supported by The German Federal Ministry for Education and Research (BMBF), No. 01GN0818 and No. 01GN0819; the Max-Planck Society, and initially by the Dr. Helmut Storz Stiftung
Correspondence to: Ahmed Mansouri, PhD, Professor, Department of Molecular Cell Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. amansou@gwdg.de
Telephone: +49-551-2011490 Fax: +49-551-2011504
Received: September 17, 2013
Revised: December 14, 2013
Accepted: January 13, 2014
Published online: April 26, 2014
Revised: December 14, 2013
Accepted: January 13, 2014
Published online: April 26, 2014
Core Tip
Core tip: The use of pluripotent cells as a source for the generation of neuronal tissue for transplantation suffers from the risk of teratoma formation. To circumvent this problem, we have developed a simple and fast protocol to obtain human neural progenitor (HNP) cell lines from embryonic stem cells. These HNP cell lines have the potential to differentiate in vitro into dopaminergic neurons. After injection into immunodeficient SCID/beige mice, they did not form tumors even after 6 mo. These findings indicate that HNP cell lines can differentiate into dopaminergic neurons and bear no risk of generating teratomas in immunodeficient mice.