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World J Stem Cells. May 26, 2011; 3(5): 43-52
Published online May 26, 2011. doi: 10.4252/wjsc.v3.i5.43
Published online May 26, 2011. doi: 10.4252/wjsc.v3.i5.43
Preclinical transplantation and safety of HS/PCs expanded from human umbilical cord blood
Chun-Juan Guo, Di Hou, Dong-Yan Shi, Yong-Mei Xi, Jin-Fu Wang, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
Ying Gao, The Second People’s Hospital of Hangzhou, Hangzhou 310015, Zhejiang Province, China
Xiang-Min Tong, Dan Shen, The First Auxiliary Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Author contributions: Guo CJ participated in the experimental design, HS/PC expansion and analysis, determination of contamination in HS/PC product, interpretation of the data and was the primary author of the manuscript; Gao Y collected human umbilical cord blood and isolated hematopoietic stem cells, and performed the engraftment assay of HS/PC products in NOD/SCID mice; Hou D conceived the experiment, performed the culture of BM-MSCs; Shi DY detected the bacterial endotoxin concentration and stem cell factor; Tong XM collected human BM; Shen D isolated MSCs from BM; Xi YM participated in manuscript preparation; Wang JF supervised the study design, execution, analysis, and approved the final version.
Supported by Scientific Foundation of Zhejiang (2009C13020) and National Natural Science Foundation of China, No. 30971460
Correspondence to: Jin-Fu Wang, Professor, Institute of Cell Biology and Genetics, College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou 310058, Zhejiang Province, China. wjfu@zju.edu.cn
Telephone: +86-571-88206592 Fax: +86-571-85128776
Received: August 10, 2010
Revised: January 5, 2011
Accepted: January 12, 2011
Published online: May 26, 2011
Revised: January 5, 2011
Accepted: January 12, 2011
Published online: May 26, 2011
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