Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Sep 26, 2022; 14(9): 714-728
Published online Sep 26, 2022. doi: 10.4252/wjsc.v14.i9.714
Long non-coding RNA SNHG16 promotes human placenta-derived mesenchymal stem cell proliferation capacity through the PI3K/AKT pathway under hypoxia
Xu-Dong Feng, Jia-Hang Zhou, Jun-Yao Chen, Bing Feng, Rui-Tian Hu, Jian Wu, Qiao-Ling Pan, Jin-Feng Yang, Jiong Yu, Hong-Cui Cao
Xu-Dong Feng, Jia-Hang Zhou, Jun-Yao Chen, Bing Feng, Jian Wu, Qiao-Ling Pan, Jin-Feng Yang, Jiong Yu, Hong-Cui Cao, State Key Laboratory for The Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, China
Rui-Tian Hu, Department of Chemistry, Duke University, Durham, NC 27708, United States
Jian Wu, Jinan Microecological Biomedicine Shandong Laboratory, Jinan 250117, Shandong Province, China
Hong-Cui Cao, Key Laboratory of Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases of Zhejiang Province, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, China
Author contributions: Feng XD contributed to the study design, experiments, data collection, and manuscript writing; Zhou JH, Chen JY, Feng B, Hu RT, and Wu J contributed to data collection and analysis; Pan QL, Yang JF and Yu J contributed to revising the manuscript; Cao HC contributed to design of the study, revision of the manuscript, and funding acquisition; All authors have read and approved the final manuscript.
Supported by Stem Cell and Translational Research from National Key Research and Development Program of China, No. 2020YFA0113003; and National Natural Science Foundation of China, No. 81971756.
Institutional review board statement: All protocols for the processing of human tissues and cells were approved by the Ethics Committee of The First Affiliated Hospital of Zhejiang University, No. 2020-1088.
Informed consent statement: Informed consent was waived.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The data presented in this study are available within the article text and figures.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Cui Cao, Professor, State Key Laboratory for The Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. hccao@zju.edu.cn
Received: May 2, 2022
Peer-review started: May 2, 2022
First decision: June 11, 2022
Revised: June 24, 2022
Accepted: August 15, 2022
Article in press: August 15, 2022
Published online: September 26, 2022
Core Tip

Core Tip: This study revealed the specific expression and co-expressed profiles of long non-coding RNAs and messenger RNAs in human placenta-derived mesenchymal stem cells under hypoxia by RNA sequencing assays. Through the performance of a series of systemic bioinformatic analyses, the hypoxia-responsive long non-coding RNA SNHG16 that may play a role in proliferation was screened out. Furthermore, through the use of molecular biology experiments, SNHG16 was found to affect human placenta-derived mesenchymal stem cell proliferation rates and cell cycle progression by activating the PI3K/AKT pathway and upregulating the expression of the key cell cycle regulators.