Published online Aug 26, 2020. doi: 10.4252/wjsc.v12.i8.841
Peer-review started: February 25, 2020
First decision: May 26, 2020
Revised: July 17, 2020
Accepted: August 1, 2020
Article in press: August 1, 2020
Published online: August 26, 2020
Core tip: Aqueous extracts (AE) generated with tobacco heating systems (THS) showed no differences in suspended particles compared to the cell culture medium. This finding supports previous studies demonstrating reduced levels of harmful constituents reported in THS AE in comparison to conventional cigarettes AE. The time to consume one unit (stick/cigarette) was longer for THS than for a conventional cigarette. The pH from both AE fractions was similar to the cell culture medium. Following a single exposure, THS AE was significantly less toxic to bone-forming cells and osteoprogenitor cells than conventional cigarettes AE. The cytotoxicity observed following THS exposure was associated with very high, non-physiological concentrations. No significant differences in cytotoxicity were observed between different flavors of THS AE. Moreover, THS AE displayed less impact on osteogenic differentiation of osteoprogenitor cells and the function of bone-forming cells when compared to conventional cigarettes AE. Finally, compared to conventional cigarettes AE, THS AE induced lower levels of oxidative stress due to the reduced level of harmful constituents, resulting in less damage to primary cilia structure and reduced impact on osteogenic differentiation.