Published online Aug 26, 2020. doi: 10.4252/wjsc.v12.i8.706
Peer-review started: May 18, 2020
First decision: June 5, 2020
Revised: July 8, 2020
Accepted: August 14, 2020
Article in press: August 14, 2020
Published online: August 26, 2020
Core tip: Current methods for determining prognosis in multiple myeloma are limited. The prototype device called Multi-Phase Laser-cavitation Single Cell Analyzer can perform reverse transcriptase polymerase chain reaction (RT-PCR) on single cells in a one-step microfluidics chip platform. The ability of the microfluidics chip platform to enrich plasma cell content by depleting CD45+ white blood cells has been demonstrated. Further studies will need to combine single-cell selection with RT-PCR to further enhance the diagnostic capabilities of this technology. This platform has the potential to be used for clinical risk stratification in multiple myeloma as well as minimal residual disease monitoring and selection of therapies to modulate the development of resistance.