Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach
Ning-Ning Guo, Li-Ping Liu, Yun-Wen Zheng, Yu-Mei Li, Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Yun-Wen Zheng, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, University of Tsukuba Faculty of Medicine, Tsukuba, Ibaraki 305-8575, Japan
Yun-Wen Zheng, Yokohama City University School of Medicine, Yokohama, Kanagawa 234-0006, Japan
Yun-Wen Zheng, Division of Regenerative Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, the University of Tokyo, Tokyo 108-8639, Japan
Author contributions: Guo NN conceived the study and drafted the manuscript; Liu LP, Zheng YW and Li YM contributed in discussions on the subject and reviewing the final manuscript text; all the authors approved the final version of the article; Guo NN and Liu LP contributed equally to this work; Li YM and Zheng YW are senior authors and co-correspondent to this work.
Supported by National Natural Science Foundation of China, No. 81770621, No. 81573053; Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI, No. 16K15604, No. 18H02866; Natural Science Foundation of Jiangsu Province, No. BK20180281.
Conflict-of-interest statement: No potential conflict of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Yun-Wen Zheng, PhD, Associate Professor, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, University of Tsukuba Faculty of Medicine,Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan. firstname.lastname@example.org
Received: April 1, 2019
Peer-review started: April 3, 2019
First decision: August 23,2019
Revised: September 30, 2019
Accepted: December 13, 2019
Article in press: December 13, 2019
Published online: January 26, 2020