Published online Aug 26, 2022. doi: 10.4252/wjsc.v14.i8.616
Peer-review started: March 5, 2022
First decision: April 19, 2022
Revised: May 2, 2022
Accepted: July 8, 2022
Article in press: July 8, 2022
Published online: August 26, 2022
Cell membrane fluidity is a critically important physical property for the regulation of cell behavior, but it has not been studied for the spheroid-forming cells so far.
In present study, we investigated the linkage between cell membrane fluidity and the morphological change from the three-dimensional (3D) spheroids generated by mesenchymal stem cells (MSCs) on various culture substrates.
We tried to unveil the interaction of cells through membrane exchange in the homogeneous MSC spheroids and the heterotypic co-spheroids of MSCs/cancer cells and co-spheroids of MSCs/fibroblasts.
We generated three-dimensional MSC spheroids on the surface of various culture substrates.
We discovered that vesicle-like bubbles randomly appeared on the outer layer of the MSC spheroids.
Current findings provide a novel direction to elucidate the complicated physiological alterations for 3D spheroid-forming cells.
These physiological changes of 3D spheroid-forming cells demonstrated that membrane fluidity may be altered significantly by direct cell-cell interaction on surfaces of biomaterial cell culture substrates. The membrane fluidity and translocation of components provide a new direction to elucidate the role of 3D cellular spheroid formation in the physical/physiological property changes of cells on the material interface.