Published online Mar 26, 2022. doi: 10.4252/wjsc.v14.i3.245
Peer-review started: November 19, 2021
First decision: December 12, 2021
Revised: January 12, 2022
Accepted: February 27, 2022
Article in press: February 27, 2022
Published online: March 26, 2022
Bone marrow (BM) suppression is one of the most common side effects of radiotherapy and the primary cause of death following exposure to irradiation. Despite concerted efforts, no definitive treatment method is available. Transplantation of adipose tissue-derived stromal cells has been proposed as a promising therapy for BM suppression.
Although adipose tissue-derived stromal cell transplantation has shown reasonable efficacy in studies of BM suppression, the therapeutic effects are controversial.
We administered and examined the effects of various amounts of adipose-derived mesenchymal stromal cells (ADSCs) in mice with radiation-induced BM suppression.
Mice were divided into three groups: Normal control group, irradiated (RT) group, and stem cell-treated group after whole-body irradiation (WBI). Mouse ADSCs were intraperitoneally transplanted either once or three times at 5 × 105 cells/200 μL. The white blood cell count and levels of plasma cytokines, BM mRNA, and BM surface markers were compared between the three groups. Human BM-derived CD34+ hematopoietic progenitor cells were co-cultured with human ADSCs (hADSCs) or incubated in the presence of hADSC conditioned media (CM) to investigate the effect on human cells in vitro.
The survival rate of mice that received one ADSC transplant was higher than that in the three-transplant group. Multiple transplants of ADSCs delayed the repopulation of BM hematopoietic stem cells. Anti-inflammatory effects and M2 polarization by intraperitoneal ADSCs might suppress erythropoiesis and induce myelopoiesis in sub-lethally RT mice.
To improve survival rates post-whole-body (WBI) irradiation, the amount of mesenchymal stromal cells should be optimized for transplantation.
We demonstrated the effects of ADSC doses on BM suppression and suggest that the mechanisms involved can determine the success of future experiments and clinical applications.