Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Aug 26, 2015; 7(7): 1010-1021
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1010
Allogenic banking of dental pulp stem cells for innovative therapeutics
Pierre-Yves Collart-Dutilleul, Franck Chaubron, John De Vos, Frédéric J Cuisinier
Pierre-Yves Collart-Dutilleul, Frédéric J Cuisinier, BioNano Laboratory EA 4203, Montpellier 1 University, 34193 Montpellier Cedex5, France
Franck Chaubron, Institut Clinident BioPharma, Biopôle Clermont-Limagne, 63360 Saint Beauzire, France
John De Vos, INSERM U1040, Genetic Instability of Human Pluripotent Stem Cells, 34197 Montpellier, France
John De Vos, CHRU Montpellier, Hospital Saint-Eloi, Institut de Recherche en Biothérapie, 34197 Montpellier, France
Author contributions: Collart-Dutilleul PY, Chaubron F, De Vos J and Cuisinier FJ solely contributed to this paper.
Conflict-of-interest statement: The authors declare that they have no conflict of interests.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Frédéric J Cuisinier, DDS, PhD, BioNano Laboratory EA 4203, Montpellier 1 University, 545 Av Pr JL Viala, 34193 Montpellier Cedex5, France.
Telephone: +33-4-11759225 Fax: +33-4-11759201
Received: October 13, 2014
Peer-review started: October 14, 2014
First decision: January 8, 2015
Revised: June 8, 2015
Accepted: June 18, 2015
Article in press: June 19, 2015
Published online: August 26, 2015

Medical research in regenerative medicine and cell-based therapy has brought encouraging perspectives for the use of stem cells in clinical trials. Multiple types of stem cells, from progenitors to pluripotent stem cells, have been investigated. Among these, dental pulp stem cells (DPSCs) are mesenchymal multipotent cells coming from the dental pulp, which is the soft tissue within teeth. They represent an interesting adult stem cell source because they are recovered in large amount in dental pulps with non-invasive techniques compared to other adult stem cell sources. DPSCs can be obtained from discarded teeth, especially wisdom teeth extracted for orthodontic reasons. To shift from promising preclinical results to therapeutic applications to human, DPSCs must be prepared in clinical grade lots and transformed into advanced therapy medicinal products (ATMP). As the production of patient-specific stem cells is costly and time-consuming, allogenic biobanking of clinical grade human leukocyte antigen (HLA)-typed DPSC lines provides efficient innovative therapeutic products. DPSC biobanks represent industrial and therapeutic innovations by using discarded biological tissues (dental pulps) as a source of mesenchymal stem cells to produce and store, in good manufacturing practice (GMP) conditions, DPSC therapeutic batches. In this review, we discuss about the challenges to transfer biological samples from a donor to HLA-typed DPSC therapeutic lots, following regulations, GMP guidelines and ethical principles. We also present some clinical applications, for which there is no efficient therapeutics so far, but that DPSCs-based ATMP could potentially treat.

Keywords: Adult stem cells, Multipotent stem cells, Cell-based therapy, Cell tissue bank

Core tip: To achieve clinical applications, stem cell-based therapy must shift from lab experimentation to clinical grade stem cells. We present here the development of advanced therapy medicinal products (ATMP) by the banking of dental pulp stem cells (DPSCs) for allogenic use. The dental pulp represents an efficient tool for industrial applications due to its accessibility after wisdom teeth extraction for orthodontic purpose. DPSC therapeutic batches can be produced in good manufacturing practice condition after human leukocyte antigen typing and stored in allogenic biobanks. We propose some clinical applications, for which there is no efficient therapeutics so far, but that DPSCs-based ATMP could potentially treat.