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Role of vasoactive intestine peptide in development of experimental acid esophagitis in rabbits
Yong Han, Hui Xu, Xiao-Fei Li, Yun-Jie Wang
Yong Han, Xiao-Fei Li, Yun-Jie Wang, Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province China
Hui Xu, Department of Neurobiology, Fourth Military Medical University, Xi'an 710033, Shaanxi Province China
Supported by National Natural Science Foundation of China, No. 30300344.
Correspondence to: Dr. Yong Han, Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province China. email@example.com
Received: February 28, 2005 Revised: March 11, 2005 Accepted: March 22, 2005 Published online: May 1, 2005
AIM: To evaluate the role of vasoactive intestine peptide(VIP) in the development of experimental acid reflux esophagitis (ARE) in rabbits.
METHODS: The ARE model was established by 30 min perfusion of 0.1 mol/L HCl (1 mL/min) 5 cm above the lower esophageal sphincter (LES) for 4 consecutive days (96 h). Mucosal pathological changes of LES were examined under microscope. LES pressure was measured, and VIP expression were determined in the esophageal tissues 48 h and 96 h after the perfusion.
RESULTS: Significant inflammatory changes were observed in the esophageal mucosa after the acid perfusion (48 and 96 h). LES pressure was(1.4±0.16 kPa vs 0.7±0.12 kPa, P<0.01) at 96 h after perfusion. No significant change was detected at 48 h after perfusion(1.6±0.11 kPa vs 1.4±0.13 kPa, P>0.05). It was(1.3±0.14 vs 1.4±0.12) in the control rabbits(96 h after pNS perfusion). Expression of VIP in animal models after perfusion(48 and 96 h) was increased significantly compared with controls.
CONCLUSION: LES pressure was reduced, and expression of VIP was increased significantly in the esophageal tissue following acid perfusion. It is suggested that VIP might play an important role in the development of ARE in rabbits.
LES压力在0.1 mol/L HCl灌注96 h后明显下降(1.4±0.16 vs 0.7±0.12)kPa, t = 13.55, P<0.01; 而在连续灌注48 h后无显著改变(1.6±0.11 vs 1.4±0.13)kPa, t = 4.54, P>0.05. 对照组(生理盐水灌注96 h后)LES压力在灌注前后未见显著改变(1.3±0.14 vs 1.4±0.12)kPa, t = 2.10, P>0.05.
酸损伤48 h, 96 h 后VIP表达较对照组明显增加(P<0.05), 48 h与94 h两组之间无明显差异(P>0.05, 表1, 图1C).
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