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Effect of electro-acupuncture at Zusanli acupiont on LESP, plasma gastrin and motilin in rats
Xiang-Dong Mu, Peng-Yan Xie, Jian-Xiang Liu, Xiao-Wei Shuai, Jiang Li
Xiang-Dong Mu, Peng-Yan Xie, Jian-Xiang Liu, Xiao-Wei Shuai, Jiang Li, Department of Gastroenterology, the First Hospital of Beijing University, Beijing 100034, China
Supported by Natural Science Foundation of BeiJing, No. 7042030.
Correspondence to: Dr. Peng-Yan Xie, 8 Xishiku Street, Department of Gastroenterology, the First Hospital of Beijing University, Beijing 100034, China. email@example.com
Received: March 6, 2005 Revised: March 11, 2005 Accepted: March 22, 2005 Published online: May 1, 2005
AIM: To investigate the influence of electro-acupuncturing (EA) at Zusanli acupoint on the pressure of lower esophageal sphincter (LESP), and the plasma levels of gastrin (GAS) and motilin (MTL) in the rats.
METHODS: Forty rats were divided into five groups: control group, non-channel non-collateral acupoint group, Zusanli acupoint group, atropine group, and atropine+Zusanli group. LESP was recorded by using three-channel perfusion manometric measurement system. Radioimmunoassay was applied to detect the plasma contents of GAS and MTL in the rats.
RESULTS: LESP increased significantly under or after EA at Zusanli acupoint in both Zusanli group and non-channel non-collateral acupoint group [Increased rate: 62.7%±27.0%, 131.0%±36.4% (0-20 min), 122.6%±25.9% (21-40 min), P<0.01]. Furthermore, the variation degrees of LESP in Zusanli acupoint group were significantly higher than that of the latter(2.34±0.69 kPa vs 1.94±0.35 kPa, 3.308±0.88 kPa vs 2.30±0.36 kPA, 3.17±0.69 kPA vs 2.22±0.40 kPa, P<0.01). LESP of atropine+Zusanli group decreased significantly after intraperitoneal injection of atropine, and restored to the basic level under EA or after EA, and the variation degrees were significantly lower than those of Zusanli group (P<0.01). GAS and MTL levels in plasma of Zusanli group were significantly higher than those of control group (P<0.01). No significant difference was found between non-channel non-collateral acupoint group and control group (P1= 0.492, P2= 0.104). GAS and MTL of atropine+Zusanli group increased significantly compared with atropine or control group (P1<0.01, P2<0.05). GAS and MTL levels in plasma were positively correlated with LESP (P<0.01, r1= 0.664, r2= 0.703).
CONCLUSION: There might be acupoint and meridian specificity in the regulatory effect of EA on LESP. Cholinergic M receptor blocker partially abolishes the effect of EA on LESP, but EA can restore the decreased LESP in the rats treated with cholinergic M receptor blocker. EA at Zusanli acupoint can increase plasma GAS and MTL levels, which are positively correlated with LESP of rats, indicating that some brain-gut peptides may play a role in the effect of EA at Zusanli acupoint.
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