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Establishment of a mouse model colonized by Helicobacter pylori
Xue-Fei Tian, Xue-Gong Fan, Yan Zhang, Yan Huang
Xue-Fei Tian, Xue-Gong Fan, Yan Zhang, Yan Huang, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Supported by: the National Natural Science Foundation of China, No. 30271171, Teacher Foundation of the National Ministry of Education, No.2000-65, Nature Scientific Foundation of Hunan Province, No. 01jjy2114 and the Scientific Research Foundation of Health Bureau of Hunan Province No. 00022..
Correspondence to: Dr. Xue-Gong Fan, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. xgfan@hotmail.com
Received: February 11, 2004 Revised: February 20, 2004 Accepted: February 24, 2004 Published online: June 15, 2004
AIM: To establish a mouse model of H. pylori infection.
METHODS: C57BL/6 strain mouse, used as an experiment animal, was inoculated orally H. pylori SS1 strain and fed in laminar flow cabinet. Inoculated mouse examined by HE stain, carbolic acid- basic fuchsin stain, immunohistochemical stain, urease test and bacteria cultivation. Both bacteria DNA from gastric tissue and H. pylori isolated from stomach were analyzed by PCR and gene sequencing.
RESULTS: The bacteria isolated from stomach had the identical shape and character compared with inoculated bacteria. Bacteria colonizing in antrun crypt and infiltration of inflammatory cells in gastric submucosa were observed. H. pylori 16SrRNA and cagA gene were amplificated successfully from gastric tissue DNA, gene sequencing results showed the complete concordance.
CONCLUSION: After inoculated into C57BL/6 mouse 2 months, H. pylori SS1 can colonize successfully the mouse stomach and cause chronic gastritis.
Key Words: N/A
Citation: Tian XF, Fan XG, Zhang Y, Huang Y. Establishment of a mouse model colonized by Helicobacter pylori. Shijie Huaren Xiaohua Zazhi 2004; 12(6): 1313-1316
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