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Effect of Chinese medicine Weikangning on expression of VEGF and its receptors Flt and KDR in gastric carcinoma cells
Cun-Yun Min, Qing-Ming Li, Wei-Kang Wu
Cun-Yun Min, Qing-Ming Li, Wei-Kang Wu, The Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China
Correspondence to: Cun-Yun Min, The Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-Sen University, 107 Yianjiang Xilu, Guangzhou 510120, Guangdong Province, China. drmincunyun888@yahoo.com.cn
Received: June 27, 2003 Revised: August 6, 2003 Accepted: August 16, 2003 Published online: March 15, 2004
AIM: To study effect of traditional Chinese medicine Weikangning (WKN) on the expression of VEGF and its receptors Flt and KDR in gastric carcinoma cells.
METHODS: We used different dosage of WKN on rats to prepare serum containing WKN.The gastric carcinoma cells were cultured in the RPMI1640 media with serum containing WKN for 48 h. The expression of VEGF, Flt-1 and KDR was determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry in gastric cancer cell lines respectively.
RESULTS: All gastric cancer cell lines analyzed expressed VEGF Flt-1 and KDR. But the expression of fVEGF (186.82±0.22, 195.35±0.45, 172.62±0.52), Flt-1 (198.44±0.44, 188.66±0.46, 197.01±0.91) in cells cultured in serum containing WKN decreased in a dose-dependent manner as compared with control (VEGF162. 78±0.58, Flt: 172.65±0.65)(P < 0.05).
CONCLUSION: VEGF and its receptors KDR and Flt-1 are expressed widely in gastric carcinoma cells and WKN can inhibit their expression.
Key Words: N/A
Citation: Min CY, Li QM, Wu WK. Effect of Chinese medicine Weikangning on expression of VEGF and its receptors Flt and KDR in gastric carcinoma cells. Shijie Huaren Xiaohua Zazhi 2004; 12(3): 533-536
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