短发夹状RNA对人肝癌细胞环氧合酶-2的抑制作用

图1 重组质粒的酶切鉴定. 1: HK SalⅠ; 2: HK PstⅠ; 3: HK; 4: WBH2 SalⅠ; 5: WBH2 PstⅠ; 6: WBH2; 7: WBH1 SalⅠ; 8: WBH1 PstⅠ; 9: WBH1; M: DL2000 Marker.

图2 人肝癌细胞的转染效率(×200). A: HepG2细胞转染后48 h, 荧光显微镜; B: HepG2细胞相同视野, 普通光线; C: Bel7402细胞转染后48 h, 荧光显微镜; D: Bel7402细胞相同视野, 普通光线.

图3 人肝癌细胞COX-2 mRNA的表达. A: HepG2; B: Bel7402; 1: 24 h WBH1转染组; 2: 48 h WBH1转染组; 3: 72 h WBH1转染组; 4: 96 h WBH1转染组; 5: 48 h空白对照组; 6: 48 h WBH2转染组; 7: 48 h HK转染组; M: marker.

图4 人肝癌细胞COX-2蛋白的表达. A: HepG2; B: Bel7402; 1: 24 h WBH1转染组; 2: 48 h WBH1转染组; 3: 72 h WBH1转染组; 4: 96 h WBH1转染组; 5: 48 h空白对照组; 6: 48 h WBH2转染组; 7: 48 h HK转染组.