Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene

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Sep 15, 2003 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Esophageal Cancer

World J Gastroenterol. Sep 15, 2003; 9(9): 1892-1896
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.1892

Table 1 Yeast two-hybrid screening using ECRG2 as baits

Yeast transformation	Transformation efficiency^a (cfu/µg library)	Transformation yield^b	His⁺, Ade⁺, Laz⁺^c	True positives
1	2.46 × 10⁵	9 × 10⁵	48	3
2	3.17 × 10⁵	1.3 × 10⁶	63	4
3	2.32 × 10⁵	8 × 10⁵	35	2
Total		3 × 10⁶	146	9

^a: Transformation efficiency (transformants/µg) = transformation yield ÷ amount of library DNA in µg).

^b: Transformation yield (total transformants) = [(colonies/plate) ÷ (volume/plate)] × [(volume of total reaction) ÷ (dilution factor)].

^c: β-galactosidase activ-ity of the positive colonies assayed by β-galactosidase filter lift assay.

Citation: Cui YP, Wang JB, Zhang XY, Bi MX, Guo LP, Lu SH. Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene. World J Gastroenterol 2003; 9(9): 1892-1896
URL: https://www.wjgnet.com/1007-9327/full/v9/i9/1892.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i9.1892