Relationship between inactivation of p16 gene and gastric carcinoma

Table 1 The primer sequence of the p16 gene

Exon	Sequence of the primer	Product of PCR (bp)	Temperature for annealing (°C)
1S	5’TCTGCGGAGAGGGGGAGAGCAG3’	280	58
1A	5’GCGCTACCTGATTCCAATTC3’
2S	5’TTCCTTTCCGTCATGCCGG 3’	394	56
2A	5’GTACAAATTCTCAGATCATCAGTCCTC3’
3S	5’GGATGTTCCACACATCTTTG3’	189	52
3A	5’ATGAAAACTACGAAAGCGGG3’

S: Sense; A: Antisense.

Table 2 p16 protein expression at GC

Hisological types	n	Positive	Negative	The frequency of p16 protein expression loss (%)
Gastric carcinoma	40	9	31	77.5
Adjacent nontumor tissue ( ≤ 3 cm)	40	18	22	55.0
Distal normal tissue (≥ 5 cm)	40	33	7	17.5