Influence of methionine/valine-depleted enteral nutrition on nucleic acid and protein metabolism in tumor-bearing rats

Table 1 Composition of amino acid solutions (g·L^-1)

Amino acids	Balanced amino acids (Group A, C)	Methionine-depleted (Group B)	Valine-depleted (Group D )
Isoleucine	5.5	5.5	5.5
Leucine	7.5	7.5	7.5
Lysine	7.0	7.0	7.0
Methionine	6.0	-	6.0
Phenylalanine	4.0	4.0	4.0
Threonine	5.0	5.0	5.0
Tryptophan	1.5	1.5	1.5
Valine	6.0	6.0	-
Arginine	6.0	6.0	6.0
Histidine	3.0	3.0	3.0
Proline	4.0	4.0	4.0
Tyrosine	1.0	1.0	1.0
Alanine	20.0	20.0	20.0
Glycine	7.5	7.5	7.5
Aspartic acid	4.0	4.0	4.0
Total amino acid	88.0	82.0	82.0
Total N	14.1	13.1	13.1

Table 2 Compositions of EN solutions (ml·L^-1)

Amino acids	Balanced amino acids (group A, C)	Methionine-depleted (group B)	Valine-depleted (group D)
Amino acid solutions	350	350	350
50% Glucose	300	300	300
20% Intralipid	100	100	100
Electrolytes, vitamine	250	250	250
Total calorie (KJ·L-1)	3513.3	3507.9	3507.9
Total N (g·L-1)	4.9	4.6	4.6
Non-protein calorie/N	122	131	131

Table 3 Incorporation (DUR, %) of ³H into nucleic acids and proteins in TB rats after treatment

Group		0.5 h	1 h	2 h	4 h
RNA	A	0.208 ± 0.002	0.300 ± 0.002	0.349 ± 0.007	0.405 ± 0.007c
	B	0.149 ± 0.012	0.249 ± 0.009	0.260 ± 0.010	0.389 ± 0.010
	C	0.200 ± 0.007	0.250 ± 0.036b	0.283 ± 0.029ac	0.326 ± 0.014c
	D	0.180 ± 0.013	0.210 ± 0.024	0.300 ± 0.034	0.320 ± 0.030b
DNA	A	0.210 ± 0.013	0.300 ± 0.020	0.339 ± 0.039	0.400 ± 0.002c
	B	0.179 ± 0.010a	0.204 ± 0.039a	0.240 ± 0.028a	0.300 ± 0.015b
	C	0.200 ± 0.011	0.250 ± 0.040	0.283 ± 0.031c	0.340 ± 0.057c
	D	0.180 ± 0.015	0.220 ± 0.024	0.308 ± 0.007	0.320 ± 0.035
Proteins	A	0.659 ± 0.055	2.410 ± 0.149	3.450 ± 0.125	4.492 ± 0.108c
	B	0.500 ± 0.020b	2.000 ± 0.203b	2.890 ± 0.090bc	3.670 ± 0.110b
	C	0.805 ± 0.098	2.510 ± 0.010	3.540 ± 0.101c	4.180 ± 0.018c
	D	0.708 ± 0.019	1.887 ± 0.020b	2.916 ± 0.085b	3.813 ± 0.076b

^aP < 0.05,

^bP < 0.01, vs group A or C.

^cNumber of rats = 2.