Construction, expression and tumor targeting of a single-chain Fv against human colorectal carcinoma

Table 1 Immune activity of ND-1scFv determined by ELISA

Sample	OD450nm (-x±s)
	CCL-187	HeLa
ND-1IgG	1.92 ± 0.28	0.20 ± 0.06
ND-1scFv	0.87 ± 0.17	0.19 ± 0.03
PBS	0.14 ± 0.03	0.13 ± 0.01

Table 2 Distribution of ^99mTc- labeled ND-1scFv in mice-bear-ing tumor (-x± s)

Tissue	T/NT value
	1 h	3 h
Blood	2.61 ± 0.97	2.16 ± 1.05
Liver	1.20 ± 0.40	1.75 ± 1.10
Spleen	2.72 ± 0.10	1.23 ± 0.65
Kidney	0.07 ± 0.05	0.26 ± 0.01
Heart	1.75 ± 0.51	1.90 ± 0.60
Lung	0.83 ± 0.31	0.62 ± 0.16

Table 3 Pharmacokinetic parameter of ^99mTc-labeled ND-1scFv in mice-bearing tumor

	ND-1IgG	ND-1scFv
Alpha half-life (min)	5.7	60
Beta half-life (h)	2.6	18