Studies on microsatellite instability in p16 gene and expression of hMSH2 mRNA in human gastric cancer tissues

Table 1 LOH at D9s171 and D9s 1604 in gastric cancer and adjacent cancer tissue

	LOH(+)	LOH(-)	Total
Gastric cancer tissue	13	7	20
Adjacent cancer tissue	6	14	20
Total	19	21	40

^aP < 0.05 vs adjacent cancer tissue.

Table 2 Relation between LOH occurred at D9s171 and at D9s1604 in gastric cancer

D9s1604	D9s171		Total
	+	-
+	4	10	14
-	1	25	26
Total	5	35	40

+: LOH (+); -: LOH (-); ^aP < 0.05 vs LOH (-).

Table 3 hMSH2 mRNA in the normal gastric mucosa, gastric cancer and adjacent cancer tissue (x±s) (In situ hybridization)

	n	No. of positive cases (ratio)	No. of positive cells in each scope
Normal gastric mucosa	19	13(68.4%)	175.8 ± 26.4a
Adjacent cancer tissue	10	6(60%)	99.7 ± 16.8b
Gastric cancer tissue	27	20(74.1%)	42.1 ± 25.9c

^aP < 0.01 vs adjacent cancer tissue;

^bP < 0.01 vs gastric cancer tissue.

Table 4 hMSH2 mRNA in various clinical stage gastric cancer (In situ hybridization)

Clinical stage	n	No. of positive cases	No. of positive cells	F value	P value
I	5	4	62.8 ± 25.4	2.495	0.097
II	10	8	46.3 ± 24.4
III	9	6	32.3 ± 22.3
IV	3	2	13.5 ± 3.5