Correlation between metastatic potential and variants from colorectal tumor cell line HT-29

Table 1 Macroscopical pattern of HT-29c/d cell metastases

Cell	Hepatic metastasis				Lung metastasis			Other organs
	No of metasta	I	II	III	No of metasta	I	II
HT-29c	1/2	0/2	0/2	1/2	1/2	1/2	0/2	2/2
HT-29d	7/10	2/10	3/10	2/10	3/10	1/10	2/10	9/10

a: metastasis.

Table 2 uPA and PAI-1 level of different cell lines at 24 h

Cell	Level (pg/105 cell)
	uPA (¯x ± s)	PAI-1 (¯x ± s)
WiDr	16.7 ± 5.8	1613 ± 90
HT-29	80.01 ± 8.1a	2650 ± 1154a
HT-29d	87.0 ± 20.8b	9707 ± 2450b

^aP < 0.05 vs WiDr,

^bP < 0.05 vs HT-29.

Table 3 Expression of CEA and PI3-Kinase in vitro and in vivo

Cell lines and tumor	CEA		PI3-Kinase
	Positive cell (%)	Intensity	Positive cell (%)	Intensity
WiDr	93 ± 4.4	+++	99 ± 0.8	++
HT-29	90 ± 2.1	+++	98 ± 1.6	++
HT-29d	94 ± 3.0	+++	97 ± 3.6	++
WiDr s.ca.	29 ± 2.6	++	14 ± 0.8	+
HT-29 s.c.	31 ± 4.6	++	22 ± 0.8	+
HT-29d s.c.	40 ± 6.0	+++	23 ± 2.2	++
HT-29 Lmb	71 ± 7.9	+++	28 ± 0.8	+
HT-29d Lm	66 ± 7.0	+++	34 ± 0.8	++

a: s.c.: s.c. tumor, b: Lm: liver metastases.