Brief Reports
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 15, 2003; 9(11): 2627-2631
Published online Nov 15, 2003. doi: 10.3748/wjg.v9.i11.2627
Correlation between metastatic potential and variants from colorectal tumor cell line HT-29
Min Wang, Ilka Vogel, Holger Kalthoff
Min Wang, Department of Surgical Oncology, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Ilka Vogel, Holger Kalthoff, Molecular Oncology Research Laboratory, Clinic for General and Thoracic Surgery, Christian-Albrechts-University, 24105 Kiel, Germany
Author contributions: All authors contributed equally to the work.
Supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, Personnel Affairs Bureau of Zhejiang Province
Correspondence to: Dr. Min Wang, Department of Surgical Oncology, First Affiliated Hospital of Medical College, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. pfeng@mail.hz.zj.cn
Telephone: +86-571-7236880 Fax: +86-571-7236628
Received: April 12, 2003
Revised: May 12, 2003
Accepted: May 19, 2003
Published online: November 15, 2003
Abstract

AIM: To evaluate the relationship between uPA, PAI-1, CEA, PI3K and metastatic potential in three colorectal tumor cell lines.

METHODS: Metastatic model in nude rats was established by variants HT-29c and HT-29d cell lines and the metastatic potential of two tumor cell variants was compared. Urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) were determined using ELISA in colorectal carcinoma WiDr, HT-29 and HT-29d cell lines with different metastatic potentials. Expression of carcinoembryonic antigen (CEA) and phosphoinositide 3-kinase (PI3-Kinase) was analyzed using immunohistochemistry (IHC) in these cell lines in vitro and in vivo. CEA expression was compared using fluorescence activated cell sorter (FACS) in vitro.

RESULTS: The number of HT-29d cells arrested in liver dramatically decreased within the initial 24 h after injection. The taking rate of liver metastases in the variant HT-29d increased as compared with parental HT-29 cells (70% versus 50%) and a variant HT-29b cells (70% versus 60%), and extensive organs were synchronously involved in metastases. The uPA concentration of variant HT-29d cell line was significantly higher than that of the non-metastatic WiDr and the low metastatic HT-29 cell lines. The variant HT-29d cells produced stronger PI3-kinase expression as compared with the non-metastatic WiDr cells and the low metastatic HT-29 cells in vivo.

CONCLUSION: The selected variant HT-29d cell exhibited an enhanced metastatic potential. The level of uPA and PAI-1 is positively correlated with the metastatic capacity of tumor cells. The expression of PI3-kinase correlates with tumor development and metastasis.

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